Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing

The objective of this study was to isolate and identify novel potential probiotic Lactobacillus using a culture method and Polymerase Chain Reaction (PCR) amplification of the 16S rRNA gene. Seventeen Lactobacillus strains were isolated from a full honey comb of the honey bee Apis dorsata using sele...

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Main Authors: Tajabadi, Naser, Mardan, Makhdzir, Abd Manap, Mohd Yazid, Mustafa, Shuhaimi
Format: Article
Language:English
Published: International Bee Research Association 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29319/1/Molecular%20identification%20of%20Lactobacillus%20spp.%20isolated%20from%20the%20honey%20comb%20of%20the%20honey%20bee%20%28Apis%20dorsata%29%20by%2016S%20rRNA%20gene%20sequencing.pdf
http://psasir.upm.edu.my/id/eprint/29319/
http://www.tandfonline.com/doi/abs/10.3896/IBRA.1.52.5.10?journalCode=tjar20
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spelling my.upm.eprints.293192016-07-13T02:26:29Z http://psasir.upm.edu.my/id/eprint/29319/ Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing Tajabadi, Naser Mardan, Makhdzir Abd Manap, Mohd Yazid Mustafa, Shuhaimi The objective of this study was to isolate and identify novel potential probiotic Lactobacillus using a culture method and Polymerase Chain Reaction (PCR) amplification of the 16S rRNA gene. Seventeen Lactobacillus strains were isolated from a full honey comb of the honey bee Apis dorsata using selective media. The 16S rRNA genes from the extracted DNA of bacterial colonies were amplified with PCR using universal bacteria primers. All bacterial 16S rRNA genes were sequenced, aligned and the distant bacteria were deposited in GenBank. The lactobacilli strains were identified as Lactobacillus spp. related-sequences (64.15%) with other abundant sequences being related to Lactobacillus kunkeei (34.85%). The findings revealed that Apis dorsata honey comb has potential to be a source of new bacteria with probiotic activities in honey bee or as natural food preservatives for the food industry. International Bee Research Association 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29319/1/Molecular%20identification%20of%20Lactobacillus%20spp.%20isolated%20from%20the%20honey%20comb%20of%20the%20honey%20bee%20%28Apis%20dorsata%29%20by%2016S%20rRNA%20gene%20sequencing.pdf Tajabadi, Naser and Mardan, Makhdzir and Abd Manap, Mohd Yazid and Mustafa, Shuhaimi (2013) Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing. Journal of Apicultural Research, 52 (5). pp. 235-241. ISSN 0021-8839; ESSN: 2078-6913 http://www.tandfonline.com/doi/abs/10.3896/IBRA.1.52.5.10?journalCode=tjar20 10.3896/IBRA.1.52.5.10
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The objective of this study was to isolate and identify novel potential probiotic Lactobacillus using a culture method and Polymerase Chain Reaction (PCR) amplification of the 16S rRNA gene. Seventeen Lactobacillus strains were isolated from a full honey comb of the honey bee Apis dorsata using selective media. The 16S rRNA genes from the extracted DNA of bacterial colonies were amplified with PCR using universal bacteria primers. All bacterial 16S rRNA genes were sequenced, aligned and the distant bacteria were deposited in GenBank. The lactobacilli strains were identified as Lactobacillus spp. related-sequences (64.15%) with other abundant sequences being related to Lactobacillus kunkeei (34.85%). The findings revealed that Apis dorsata honey comb has potential to be a source of new bacteria with probiotic activities in honey bee or as natural food preservatives for the food industry.
format Article
author Tajabadi, Naser
Mardan, Makhdzir
Abd Manap, Mohd Yazid
Mustafa, Shuhaimi
spellingShingle Tajabadi, Naser
Mardan, Makhdzir
Abd Manap, Mohd Yazid
Mustafa, Shuhaimi
Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing
author_facet Tajabadi, Naser
Mardan, Makhdzir
Abd Manap, Mohd Yazid
Mustafa, Shuhaimi
author_sort Tajabadi, Naser
title Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing
title_short Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing
title_full Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing
title_fullStr Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing
title_full_unstemmed Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing
title_sort molecular identification of lactobacillus spp. isolated from the honey comb of the honey bee (apis dorsata) by 16s rrna gene sequencing
publisher International Bee Research Association
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29319/1/Molecular%20identification%20of%20Lactobacillus%20spp.%20isolated%20from%20the%20honey%20comb%20of%20the%20honey%20bee%20%28Apis%20dorsata%29%20by%2016S%20rRNA%20gene%20sequencing.pdf
http://psasir.upm.edu.my/id/eprint/29319/
http://www.tandfonline.com/doi/abs/10.3896/IBRA.1.52.5.10?journalCode=tjar20
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