Characterization of cellulolytic bacterial cultures grown in different substrates

Nine aerobic cellulolytic bacterial cultures were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Culture (DSMZ) and the American Type Culture Collection (ATCC). The objectives of this study were to characterize the cellulolytic bacteria and to determine the opt...

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Main Authors: Alshelmani, Mohamed Idris, Loh, Teck Chwen, Foo, Hooi Ling, Lau, Wei Hong, Sazili, Awis Qurni
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29494/1/Characterization%20of%20cellulolytic%20bacterial%20cultures%20grown%20in%20different%20substrates.pdf
http://psasir.upm.edu.my/id/eprint/29494/
http://dx.doi.org/10.1155/2013/689235
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Institution: Universiti Putra Malaysia
Language: English
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spelling my.upm.eprints.294942016-02-12T03:33:43Z http://psasir.upm.edu.my/id/eprint/29494/ Characterization of cellulolytic bacterial cultures grown in different substrates Alshelmani, Mohamed Idris Loh, Teck Chwen Foo, Hooi Ling Lau, Wei Hong Sazili, Awis Qurni Nine aerobic cellulolytic bacterial cultures were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Culture (DSMZ) and the American Type Culture Collection (ATCC). The objectives of this study were to characterize the cellulolytic bacteria and to determine the optimum moisture ratio required for solid state fermentation (SSF) of palm kernel cake (PKC). The bacteria cultures were grown on reconstituted nutrient broth, incubated at 30∘C and agitated at 200 rpm. Carboxymethyl cellulase, xylanase, and mannanase activities were determined using different substrates and after SSF of PKC. The SSF was conducted for 4 and 7 days with inoculum size of 10% (v/w) on different PKC concentration-to-moisture ratios: 1 : 0.2, 1 : 0.3, 1 : 0.4, and 1 : 0.5. Results showed that Bacillus amyloliquefaciens 1067 DSMZ, Bacillus megaterium 9885 ATCC, Paenibacillus curdlanolyticus 10248 DSMZ, and Paenibacillus polymyxa 842 ATCC produced higher enzyme activities as compared to other bacterial cultures grown on different substrates. The cultures mentioned above also produced higher enzyme activities when they were incubated under SSF using PKC as a substrate in different PKC-to-moisture ratios after 4 days of incubation, indicating that these cellulolytic bacteria can be used to degrade and improve the nutrient quality of PKC. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29494/1/Characterization%20of%20cellulolytic%20bacterial%20cultures%20grown%20in%20different%20substrates.pdf Alshelmani, Mohamed Idris and Loh, Teck Chwen and Foo, Hooi Ling and Lau, Wei Hong and Sazili, Awis Qurni (2013) Characterization of cellulolytic bacterial cultures grown in different substrates. The Scientific World Journal, 2013. art. no. 689235. pp. 1-6. ISSN 2356-6140; ESSN: 1537-744X http://dx.doi.org/10.1155/2013/689235 10.1155/2013/689235
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Nine aerobic cellulolytic bacterial cultures were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Culture (DSMZ) and the American Type Culture Collection (ATCC). The objectives of this study were to characterize the cellulolytic bacteria and to determine the optimum moisture ratio required for solid state fermentation (SSF) of palm kernel cake (PKC). The bacteria cultures were grown on reconstituted nutrient broth, incubated at 30∘C and agitated at 200 rpm. Carboxymethyl cellulase, xylanase, and mannanase activities were determined using different substrates and after SSF of PKC. The SSF was conducted for 4 and 7 days with inoculum size of 10% (v/w) on different PKC concentration-to-moisture ratios: 1 : 0.2, 1 : 0.3, 1 : 0.4, and 1 : 0.5. Results showed that Bacillus amyloliquefaciens 1067 DSMZ, Bacillus megaterium 9885 ATCC, Paenibacillus curdlanolyticus 10248 DSMZ, and Paenibacillus polymyxa 842 ATCC produced higher enzyme activities as compared to other bacterial cultures grown on different substrates. The cultures mentioned above also produced higher enzyme activities when they were incubated under SSF using PKC as a substrate in different PKC-to-moisture ratios after 4 days of incubation, indicating that these cellulolytic bacteria can be used to degrade and improve the nutrient quality of PKC.
format Article
author Alshelmani, Mohamed Idris
Loh, Teck Chwen
Foo, Hooi Ling
Lau, Wei Hong
Sazili, Awis Qurni
spellingShingle Alshelmani, Mohamed Idris
Loh, Teck Chwen
Foo, Hooi Ling
Lau, Wei Hong
Sazili, Awis Qurni
Characterization of cellulolytic bacterial cultures grown in different substrates
author_facet Alshelmani, Mohamed Idris
Loh, Teck Chwen
Foo, Hooi Ling
Lau, Wei Hong
Sazili, Awis Qurni
author_sort Alshelmani, Mohamed Idris
title Characterization of cellulolytic bacterial cultures grown in different substrates
title_short Characterization of cellulolytic bacterial cultures grown in different substrates
title_full Characterization of cellulolytic bacterial cultures grown in different substrates
title_fullStr Characterization of cellulolytic bacterial cultures grown in different substrates
title_full_unstemmed Characterization of cellulolytic bacterial cultures grown in different substrates
title_sort characterization of cellulolytic bacterial cultures grown in different substrates
publisher Hindawi Publishing Corporation
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29494/1/Characterization%20of%20cellulolytic%20bacterial%20cultures%20grown%20in%20different%20substrates.pdf
http://psasir.upm.edu.my/id/eprint/29494/
http://dx.doi.org/10.1155/2013/689235
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