Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release

The long-term objective of the present study was to determine the ability of NiZn ferrite nanoparticles to kill cancer cells. NiZn ferrite nanoparticle suspensions were found to have an average hydrodynamic diameter, polydispersity index, and zeta potential of 254.2±29.8nm, 0.524 ±0.013, and -60±14m...

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Main Authors: Al-Qubaisi, Mothanna Sadiq, Abdullah, Rasedee, Flaifel, Moayad Husein, Ahmad, Sahrim, Al Ali, Samer Hassein, Hussein, Mohd Zobir, Zainal, Zulkarnain, Alhassan, Fatah H., Yap, Taufiq Yun Hin, Eid, Eltayeb E. M., Arbab, Ismail Adam, Al-Asbahi, Bandar A., Webster, Thomas J., El Zowalaty, Mohamed Ezzat
Format: Article
Language:English
Published: Dove Medical Press 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30109/1/Induction%20of%20apoptosis%20in%20cancer%20cells%20by%20NiZn%20ferrite%20nanoparticles%20through%20mitochondrial%20cytochrome%20C%20release.pdf
http://psasir.upm.edu.my/id/eprint/30109/
https://www.dovepress.com/induction-of-apoptosis-in-cancer-cells-by-nizn-ferrite-nanoparticles-t-peer-reviewed-article-IJN
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spelling my.upm.eprints.301092016-11-24T08:12:31Z http://psasir.upm.edu.my/id/eprint/30109/ Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release Al-Qubaisi, Mothanna Sadiq Abdullah, Rasedee Flaifel, Moayad Husein Ahmad, Sahrim Al Ali, Samer Hassein Hussein, Mohd Zobir Zainal, Zulkarnain Alhassan, Fatah H. Yap, Taufiq Yun Hin Eid, Eltayeb E. M. Arbab, Ismail Adam Al-Asbahi, Bandar A. Webster, Thomas J. El Zowalaty, Mohamed Ezzat The long-term objective of the present study was to determine the ability of NiZn ferrite nanoparticles to kill cancer cells. NiZn ferrite nanoparticle suspensions were found to have an average hydrodynamic diameter, polydispersity index, and zeta potential of 254.2±29.8nm, 0.524 ±0.013, and -60±14mV, respectively. We showed that NiZn ferrite nanoparticles had selective toxicity towards MCF-7, HepG2, and HT29cells, with a lesser effect on normal MCF 10A cells. The quantity of Bcl-2, Bax, p53, and cytochrome C in the cell lines mentioned above was determined by colorimetric methods in order to clarify the mechanism of action of NiZn ferrite nanoparticles in the killing of cancer cells. Our results indicate that NiZn ferrite nanoparticles promote apoptosis in cancer cells via caspase-3 and caspase-9, downregulation of Bcl-2, and upregulation of Bax and p53, with cytochrome C translocation. There was a concomitant collapse of the mitochondrial membrane potential in these cancer cells when treated with NiZn ferrite nanoparticles. This study shows that NiZn ferrite nanoparticles induce glutathione depletion in cancer cells, which results in increased production of reactive oxygen species and eventually, death of cancer cells. Dove Medical Press 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30109/1/Induction%20of%20apoptosis%20in%20cancer%20cells%20by%20NiZn%20ferrite%20nanoparticles%20through%20mitochondrial%20cytochrome%20C%20release.pdf Al-Qubaisi, Mothanna Sadiq and Abdullah, Rasedee and Flaifel, Moayad Husein and Ahmad, Sahrim and Al Ali, Samer Hassein and Hussein, Mohd Zobir and Zainal, Zulkarnain and Alhassan, Fatah H. and Yap, Taufiq Yun Hin and Eid, Eltayeb E. M. and Arbab, Ismail Adam and Al-Asbahi, Bandar A. and Webster, Thomas J. and El Zowalaty, Mohamed Ezzat (2013) Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release. International Journal of Nanomedicine, 8 (1). pp. 4115-4130. ISSN 1176-9114; ESSN: 1178-2013 https://www.dovepress.com/induction-of-apoptosis-in-cancer-cells-by-nizn-ferrite-nanoparticles-t-peer-reviewed-article-IJN 10.2147/IJN.S50061
institution Universiti Putra Malaysia
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country Malaysia
content_provider Universiti Putra Malaysia
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url_provider http://psasir.upm.edu.my/
language English
description The long-term objective of the present study was to determine the ability of NiZn ferrite nanoparticles to kill cancer cells. NiZn ferrite nanoparticle suspensions were found to have an average hydrodynamic diameter, polydispersity index, and zeta potential of 254.2±29.8nm, 0.524 ±0.013, and -60±14mV, respectively. We showed that NiZn ferrite nanoparticles had selective toxicity towards MCF-7, HepG2, and HT29cells, with a lesser effect on normal MCF 10A cells. The quantity of Bcl-2, Bax, p53, and cytochrome C in the cell lines mentioned above was determined by colorimetric methods in order to clarify the mechanism of action of NiZn ferrite nanoparticles in the killing of cancer cells. Our results indicate that NiZn ferrite nanoparticles promote apoptosis in cancer cells via caspase-3 and caspase-9, downregulation of Bcl-2, and upregulation of Bax and p53, with cytochrome C translocation. There was a concomitant collapse of the mitochondrial membrane potential in these cancer cells when treated with NiZn ferrite nanoparticles. This study shows that NiZn ferrite nanoparticles induce glutathione depletion in cancer cells, which results in increased production of reactive oxygen species and eventually, death of cancer cells.
format Article
author Al-Qubaisi, Mothanna Sadiq
Abdullah, Rasedee
Flaifel, Moayad Husein
Ahmad, Sahrim
Al Ali, Samer Hassein
Hussein, Mohd Zobir
Zainal, Zulkarnain
Alhassan, Fatah H.
Yap, Taufiq Yun Hin
Eid, Eltayeb E. M.
Arbab, Ismail Adam
Al-Asbahi, Bandar A.
Webster, Thomas J.
El Zowalaty, Mohamed Ezzat
spellingShingle Al-Qubaisi, Mothanna Sadiq
Abdullah, Rasedee
Flaifel, Moayad Husein
Ahmad, Sahrim
Al Ali, Samer Hassein
Hussein, Mohd Zobir
Zainal, Zulkarnain
Alhassan, Fatah H.
Yap, Taufiq Yun Hin
Eid, Eltayeb E. M.
Arbab, Ismail Adam
Al-Asbahi, Bandar A.
Webster, Thomas J.
El Zowalaty, Mohamed Ezzat
Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release
author_facet Al-Qubaisi, Mothanna Sadiq
Abdullah, Rasedee
Flaifel, Moayad Husein
Ahmad, Sahrim
Al Ali, Samer Hassein
Hussein, Mohd Zobir
Zainal, Zulkarnain
Alhassan, Fatah H.
Yap, Taufiq Yun Hin
Eid, Eltayeb E. M.
Arbab, Ismail Adam
Al-Asbahi, Bandar A.
Webster, Thomas J.
El Zowalaty, Mohamed Ezzat
author_sort Al-Qubaisi, Mothanna Sadiq
title Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release
title_short Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release
title_full Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release
title_fullStr Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release
title_full_unstemmed Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release
title_sort induction of apoptosis in cancer cells by nizn ferrite nanoparticles through mitochondrial cytochrome c release
publisher Dove Medical Press
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/30109/1/Induction%20of%20apoptosis%20in%20cancer%20cells%20by%20NiZn%20ferrite%20nanoparticles%20through%20mitochondrial%20cytochrome%20C%20release.pdf
http://psasir.upm.edu.my/id/eprint/30109/
https://www.dovepress.com/induction-of-apoptosis-in-cancer-cells-by-nizn-ferrite-nanoparticles-t-peer-reviewed-article-IJN
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