Preventive inositol hexaphosphate extracted from rice bran inhibits colorectal cancer through involvement of Wnt/β-catenin and COX-2 pathways

• Main Authors: Shafie, Nurul Husna, Mohd Esa, Norhaizan, Ithnin, Hairuszah, Md. Akim, Abdah, Saad, Norazalina, Pandurangan, Ashok Kumar
• Format: Article
• Language: English
• Published: Hindawi Publishing Corporation 2013
• Online Access: http://psasir.upm.edu.my/id/eprint/30727/1/Preventive%20inositol%20hexaphosphate%20extracted%20from%20rice%20bran%20inhibits%20colorectal%20cancer%20through%20involvement%20of%20Wnt.pdf; http://psasir.upm.edu.my/id/eprint/30727/; http://www.hindawi.com/journals/bmri/2013/681027/
• Institution: Universiti Putra Malaysia

Nutritional or dietary factors have drawn attention due to their potential as an effective chemopreventive agent, which is considered a more rational strategy in cancer treatment. This study was designed to evaluate the effect of IP₆ extracted from rice bran on azoxymethane- (AOM-) induced colorectal cancer (CRC) in rats. Initially, male Sprague Dawley rats were divided into 5 groups, with 6 rats in each group. The rats received two intraperitoneal (i.p.) injections of AOM in saline (15 mg/kg body weight) over a 2-week period to induce CRC. IP₆ was given in three concentrations, 0.2% (w/v), 0.5% (w/v), and 1.0% (w/v), via drinking water for 16 weeks. The deregulation of the Wnt/β-catenin signaling pathway and the expression of cyclooxygenase (COX)-2 have been implicated in colorectal tumorigenesis. β-Catenin and COX-2 expressions were analysed using the quantitative RT-PCR and Western blotting. Herein, we reported that the administration of IP₆ markedly suppressed the incidence of tumors when compared to the control. Interestingly, the administration of IP₆ had also markedly decreased β-catenin and COX-2 in colon tumors. Thus, the downregulation of β-catenin and COX-2 could play a role in inhibiting the CRC development induced by IP₆ and thereby act as a potent anticancer agent.