The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids

• Main Authors: Abdul Samad, Norani, Ari, Zainab
• Format: Article
• Language: English
• Published: Universiti Putra Malaysia Press 1993
• Online Access: http://psasir.upm.edu.my/id/eprint/3186/1/The_Use_of_Antibody-sensitized_Latex_to_Detect_Cymbidium_Mosaic.pdf; http://psasir.upm.edu.my/id/eprint/3186/; http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2016%20(2)%20Aug.%201993/13%20JTAS%20Vol.16%20(2)%201993%20(Pg%20157-160).pdf
• Institution: Universiti Putra Malaysia

Latex agglutination test (L) and a modification of the latex test, protein A-coated latex (PAL) were compared for sensitization with antibodies to Cymbidium mosaic virus (CyMV). A series of globulin concentrations was tested against purified CyMV. The minimum detectable virus concentration was similar for both L and PAL reactions at 0.104 llg/ml. The detectable end points in infected Oncidium Gower Ramsey orchid sap were similar at a dilution of 1/2560. PAL was found to be more sensitive than L at a higher globulin dilution of 1/512. The PAL test showed higher sensitivity than L based on five orchid hybrids and compared favourably with electron microscopy test.