A method for control proteolytic degradation of recombinant proteins.
The present invention provides a method to enhance the recovery of recombinant N protein of Nipah virus produced in Escherichia coli, in which by identifying and inhibiting the specific proteases in the cell lysate are achieved by an integrated use of a bioinformatics tool and a protease inhibition...
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| Main Authors: | , , |
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| Format: | Patent |
| Language: | English |
| Published: |
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/32100/ |
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| Institution: | Universiti Putra Malaysia |
| Language: | English |
| Summary: | The present invention provides a method to enhance the recovery of recombinant N protein of Nipah virus produced in Escherichia coli, in which by identifying and inhibiting the specific proteases in the cell lysate are achieved by an integrated use of a bioinformatics tool and a protease inhibition experimental setup.
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