Comparison of arbitrarily primed PCR, antibiotic resistance and plasmid profiling for differentiating Salmonella enteritidis isolated from fish

A total of 28 isolates of Salmonella enteritidis isolated from Tilapia (Tilapia mossambica) were investigated through arbitrarily primed polymerase chain reaction (AP-PCR) fingerprinting using three primers, plasmid profiling and their antibiotic resistance patterns. All 28 isolates carried at least...

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Main Authors: Rodu, Son, Abd. Mutalib, Sahilah, Rahmat Ali, Gulam Rusul, Lihan, Samuel, Mat Issa @ Zakaria, Zuraini, Elhadi, Nasreldin
Format: Article
Language:English
Published: Asian Fisheries Society 2000
Online Access:http://psasir.upm.edu.my/id/eprint/32932/1/63.%20Comparison%20of%20Arbitrarily%20Primed%20PCR%2C%20Antibiotic%20Resistance%20and%20Plasmid%20Profiling%20for%20Differentiating%20Salmonella%20enteritidis%20Isolated%20from%20Fish.pdf
http://psasir.upm.edu.my/id/eprint/32932/
http://www.asianfisheriessociety.org/publication/downloadfile.php?id=424&file=WTBkU2JVeDZRVEZOUkdzMVQwUmpkMDFFUlhwT1ZGVTBUV3BOZWsxVVNYVmpSMUp0VFdwQmVFNXFSWGhOVkZVOQ==&dldname=Comparison%20of%20Arbitrarily%20Primed%20PCR,%20Antibiotic%20Resistance%20and%20
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Institution: Universiti Putra Malaysia
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spelling my.upm.eprints.329322016-11-15T05:14:33Z http://psasir.upm.edu.my/id/eprint/32932/ Comparison of arbitrarily primed PCR, antibiotic resistance and plasmid profiling for differentiating Salmonella enteritidis isolated from fish Rodu, Son Abd. Mutalib, Sahilah Rahmat Ali, Gulam Rusul Lihan, Samuel Mat Issa @ Zakaria, Zuraini Elhadi, Nasreldin A total of 28 isolates of Salmonella enteritidis isolated from Tilapia (Tilapia mossambica) were investigated through arbitrarily primed polymerase chain reaction (AP-PCR) fingerprinting using three primers, plasmid profiling and their antibiotic resistance patterns. All 28 isolates carried at least one plasmid ranging in size from 1.4 to 38 megadalton that enabled the S. enteritidis to be grouped into nine plasmid profiles. Isolates were resistant to nalidixic acid (21.1%), penicillin (39.3%) and streptomycin (71.4%), and were susceptible to carbenicillin, cephalothin, kanamycin, rifampicin and tetracycline. Seventeen isolates (60.7%) were resistant to a single antibiotic and eleven (39.3%) were resistant to two antibiotics; thus separating the isolates into six antibiotic resistance patterns. The AP-PCR results showed that the collection of isolates were genetically very heterogenous. AP-PCR enab led us to differentiate the isolates into 28 AP-PCR types. Analysis derived from these data in combination (AP-PCR, plasmid profiling and antibiotic resistance patterns) showed that all 28 isolates were distinct and were grouped into 28 individual groups. Our results demonstrate that AP-PCR fingerprinting method is more sensitive than plasmid profiling and antibiotic resistance patterns with respect to the individualization of the isolates used in this study. Asian Fisheries Society 2000 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/32932/1/63.%20Comparison%20of%20Arbitrarily%20Primed%20PCR%2C%20Antibiotic%20Resistance%20and%20Plasmid%20Profiling%20for%20Differentiating%20Salmonella%20enteritidis%20Isolated%20from%20Fish.pdf Rodu, Son and Abd. Mutalib, Sahilah and Rahmat Ali, Gulam Rusul and Lihan, Samuel and Mat Issa @ Zakaria, Zuraini and Elhadi, Nasreldin (2000) Comparison of arbitrarily primed PCR, antibiotic resistance and plasmid profiling for differentiating Salmonella enteritidis isolated from fish. Asian Fisheries Science, 13 (1). pp. 13-20. ISSN 0116-6514 http://www.asianfisheriessociety.org/publication/downloadfile.php?id=424&file=WTBkU2JVeDZRVEZOUkdzMVQwUmpkMDFFUlhwT1ZGVTBUV3BOZWsxVVNYVmpSMUp0VFdwQmVFNXFSWGhOVkZVOQ==&dldname=Comparison%20of%20Arbitrarily%20Primed%20PCR,%20Antibiotic%20Resistance%20and%20
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A total of 28 isolates of Salmonella enteritidis isolated from Tilapia (Tilapia mossambica) were investigated through arbitrarily primed polymerase chain reaction (AP-PCR) fingerprinting using three primers, plasmid profiling and their antibiotic resistance patterns. All 28 isolates carried at least one plasmid ranging in size from 1.4 to 38 megadalton that enabled the S. enteritidis to be grouped into nine plasmid profiles. Isolates were resistant to nalidixic acid (21.1%), penicillin (39.3%) and streptomycin (71.4%), and were susceptible to carbenicillin, cephalothin, kanamycin, rifampicin and tetracycline. Seventeen isolates (60.7%) were resistant to a single antibiotic and eleven (39.3%) were resistant to two antibiotics; thus separating the isolates into six antibiotic resistance patterns. The AP-PCR results showed that the collection of isolates were genetically very heterogenous. AP-PCR enab led us to differentiate the isolates into 28 AP-PCR types. Analysis derived from these data in combination (AP-PCR, plasmid profiling and antibiotic resistance patterns) showed that all 28 isolates were distinct and were grouped into 28 individual groups. Our results demonstrate that AP-PCR fingerprinting method is more sensitive than plasmid profiling and antibiotic resistance patterns with respect to the individualization of the isolates used in this study.
format Article
author Rodu, Son
Abd. Mutalib, Sahilah
Rahmat Ali, Gulam Rusul
Lihan, Samuel
Mat Issa @ Zakaria, Zuraini
Elhadi, Nasreldin
spellingShingle Rodu, Son
Abd. Mutalib, Sahilah
Rahmat Ali, Gulam Rusul
Lihan, Samuel
Mat Issa @ Zakaria, Zuraini
Elhadi, Nasreldin
Comparison of arbitrarily primed PCR, antibiotic resistance and plasmid profiling for differentiating Salmonella enteritidis isolated from fish
author_facet Rodu, Son
Abd. Mutalib, Sahilah
Rahmat Ali, Gulam Rusul
Lihan, Samuel
Mat Issa @ Zakaria, Zuraini
Elhadi, Nasreldin
author_sort Rodu, Son
title Comparison of arbitrarily primed PCR, antibiotic resistance and plasmid profiling for differentiating Salmonella enteritidis isolated from fish
title_short Comparison of arbitrarily primed PCR, antibiotic resistance and plasmid profiling for differentiating Salmonella enteritidis isolated from fish
title_full Comparison of arbitrarily primed PCR, antibiotic resistance and plasmid profiling for differentiating Salmonella enteritidis isolated from fish
title_fullStr Comparison of arbitrarily primed PCR, antibiotic resistance and plasmid profiling for differentiating Salmonella enteritidis isolated from fish
title_full_unstemmed Comparison of arbitrarily primed PCR, antibiotic resistance and plasmid profiling for differentiating Salmonella enteritidis isolated from fish
title_sort comparison of arbitrarily primed pcr, antibiotic resistance and plasmid profiling for differentiating salmonella enteritidis isolated from fish
publisher Asian Fisheries Society
publishDate 2000
url http://psasir.upm.edu.my/id/eprint/32932/1/63.%20Comparison%20of%20Arbitrarily%20Primed%20PCR%2C%20Antibiotic%20Resistance%20and%20Plasmid%20Profiling%20for%20Differentiating%20Salmonella%20enteritidis%20Isolated%20from%20Fish.pdf
http://psasir.upm.edu.my/id/eprint/32932/
http://www.asianfisheriessociety.org/publication/downloadfile.php?id=424&file=WTBkU2JVeDZRVEZOUkdzMVQwUmpkMDFFUlhwT1ZGVTBUV3BOZWsxVVNYVmpSMUp0VFdwQmVFNXFSWGhOVkZVOQ==&dldname=Comparison%20of%20Arbitrarily%20Primed%20PCR,%20Antibiotic%20Resistance%20and%20
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