A process for producing lipase
A process for producing lipase by microorganisms on the industrial scale, wherein Escherichia coli microorganism harbouring lipase, preferably thermostable lipase, is fermented under batch or fed batch conditions, in a Luria Bertani (LB) broth supplemented with citric acid, (NH4)2SO4, Na2HPO4.12H2O,...
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2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/33645/ |
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my.upm.eprints.336452016-09-27T01:19:01Z http://psasir.upm.edu.my/id/eprint/33645/ A process for producing lipase Raja Abdul Rahman, Raja Noor Zaliha A process for producing lipase by microorganisms on the industrial scale, wherein Escherichia coli microorganism harbouring lipase, preferably thermostable lipase, is fermented under batch or fed batch conditions, in a Luria Bertani (LB) broth supplemented with citric acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, NH4Cl and glucose, using a trace metal solution containing hydrochloric acid, FeSO4.7H2O, ZnSO4.7H20, CaCl2.H20, MnSO4.5H20, CuSO4.5H2O, AlCl.6H2O, and H3BO3, and a feeding nutrient solution containing glucose, yeast extract, MgSO4.H2O, KH2PO4, Citric Acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, and NH4Cl, at dissolved oxygen tension of 30-50 %, at temperature of 30-40 °C, while maintaining the pH between 6 and 8, with a glucose content of 15-25 g/L, with isopropylthiogalactoside. 2013-05-20 Patent NonPeerReviewed Raja Noor Zaliha Raja Abd. Rahman (2013) A process for producing lipase. PI2013700818. |
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| description |
A process for producing lipase by microorganisms on the industrial scale, wherein Escherichia coli microorganism harbouring lipase, preferably thermostable lipase, is fermented under batch or fed batch conditions, in a Luria Bertani (LB) broth supplemented with citric acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, NH4Cl and glucose, using a trace metal solution containing hydrochloric acid, FeSO4.7H2O, ZnSO4.7H20, CaCl2.H20, MnSO4.5H20, CuSO4.5H2O, AlCl.6H2O, and H3BO3, and a feeding nutrient solution containing glucose, yeast extract, MgSO4.H2O, KH2PO4, Citric Acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, and NH4Cl, at dissolved oxygen tension of 30-50 %, at temperature of 30-40 °C, while maintaining the pH between 6 and 8, with a glucose content of 15-25 g/L, with isopropylthiogalactoside. |
| format |
Patent |
| author |
Raja Abdul Rahman, Raja Noor Zaliha |
| spellingShingle |
Raja Abdul Rahman, Raja Noor Zaliha A process for producing lipase |
| author_facet |
Raja Abdul Rahman, Raja Noor Zaliha |
| author_sort |
Raja Abdul Rahman, Raja Noor Zaliha |
| title |
A process for producing lipase |
| title_short |
A process for producing lipase |
| title_full |
A process for producing lipase |
| title_fullStr |
A process for producing lipase |
| title_full_unstemmed |
A process for producing lipase |
| title_sort |
process for producing lipase |
| publishDate |
2013 |
| url |
http://psasir.upm.edu.my/id/eprint/33645/ |
| _version_ |
1643830930883739648 |