Loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus
The present invention provides a loop-mediated isothermal amplification (LAMP) method for the detection of infectious spleen and kidney necrosis virus (ISKNV) infection by amplifying a portion of ISKNV major capsid protein (MCP) gene using isothermal amplification and detecting the amplified product...
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2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/33752/ |
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my.upm.eprints.337522015-03-31T01:01:13Z http://psasir.upm.edu.my/id/eprint/33752/ Loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus Mohamed Din, Mohamed Shariff Subramanian, Kuttichantran Bejo, Mohd Hair Omar, Abdul Rahman Ong, Bee Lee The present invention provides a loop-mediated isothermal amplification (LAMP) method for the detection of infectious spleen and kidney necrosis virus (ISKNV) infection by amplifying a portion of ISKNV major capsid protein (MCP) gene using isothermal amplification and detecting the amplified product by acridine orange. Newly designed primers for use with this specific method are provided as well.The LAMP assay of the invention is specific towards ISKNV and there was no cross-amplification with other pathogens including the RSIV. 2013-10-29 Patent NonPeerReviewed Mohamed Shariff Mohamed Din (2013) Loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus. PI2013702063. |
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| description |
The present invention provides a loop-mediated isothermal amplification (LAMP) method for the detection of infectious spleen and kidney necrosis virus (ISKNV) infection by amplifying a portion of ISKNV major capsid protein (MCP) gene using isothermal amplification and detecting the amplified product by acridine orange. Newly designed primers for use with this specific method are provided as well.The LAMP assay of the invention is specific towards ISKNV and there was no cross-amplification with other pathogens including the RSIV. |
| format |
Patent |
| author |
Mohamed Din, Mohamed Shariff Subramanian, Kuttichantran Bejo, Mohd Hair Omar, Abdul Rahman Ong, Bee Lee |
| spellingShingle |
Mohamed Din, Mohamed Shariff Subramanian, Kuttichantran Bejo, Mohd Hair Omar, Abdul Rahman Ong, Bee Lee Loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus |
| author_facet |
Mohamed Din, Mohamed Shariff Subramanian, Kuttichantran Bejo, Mohd Hair Omar, Abdul Rahman Ong, Bee Lee |
| author_sort |
Mohamed Din, Mohamed Shariff |
| title |
Loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus |
| title_short |
Loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus |
| title_full |
Loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus |
| title_fullStr |
Loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus |
| title_full_unstemmed |
Loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus |
| title_sort |
loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus |
| publishDate |
2013 |
| url |
http://psasir.upm.edu.my/id/eprint/33752/ |
| _version_ |
1643830961623793664 |