Intracellular production of IFN-alpha 2b in Lactococcus lactis
Human interferon alpha (IFN-α) was expressed in two strains of Lactococcus lactis by aid of two promoters (P32 and Pnis) giving rise to two recombinant strains: MG:IFN and NZ:IFN, respectively. The expression of IFN was confirmed by ELISA and western blotting. Highest production was achieved using g...
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Main Authors: | , , , , |
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Format: | Article |
Published: |
Springer Netherlands
2014
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Online Access: | http://psasir.upm.edu.my/id/eprint/34246/ http://link.springer.com/article/10.1007/s10529-013-1390-4 |
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Institution: | Universiti Putra Malaysia |