Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique

A simple and rapid method of DNA microsatellite isolation based on the Random Amplified Microsatellites (RAMs) PCR technique was used in this study. The work presented here is part of a continuous effort in refining and perfecting the technique for more rapid, effective and optimum productivity in s...

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Main Authors: Chan, Soon Choy, Siraj, Siti Shapor, Tan, Soon Guan, Mohd Yusoff, Khatijah
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2005
Online Access:http://psasir.upm.edu.my/id/eprint/3656/1/Rapid_and_Non-radioactive_Detection_Method_of_Microsatellites_in_Mystus.pdf
http://psasir.upm.edu.my/id/eprint/3656/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2028%20(1)%20Apr.%202005/JTAS%20Vol.28%20(1)%202005%20(73-77).pdf
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Institution: Universiti Putra Malaysia
Language: English
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spelling my.upm.eprints.36562016-12-02T02:44:07Z http://psasir.upm.edu.my/id/eprint/3656/ Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique Chan, Soon Choy Siraj, Siti Shapor Tan, Soon Guan Mohd Yusoff, Khatijah A simple and rapid method of DNA microsatellite isolation based on the Random Amplified Microsatellites (RAMs) PCR technique was used in this study. The work presented here is part of a continuous effort in refining and perfecting the technique for more rapid, effective and optimum productivity in single locus microsatellite marker development for the River catfish, Mystus nemurus. The current refined protocol for microsatellite isolation was able to detect a total of 135 microsatellite regions resulting in 42 unique genomic sequences being submitted to GenBank. This refined technique is able to reduce the total time required from peR cloning till sequencing specific microsatellite regions to less than three and a half months. Universiti Putra Malaysia Press 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/3656/1/Rapid_and_Non-radioactive_Detection_Method_of_Microsatellites_in_Mystus.pdf Chan, Soon Choy and Siraj, Siti Shapor and Tan, Soon Guan and Mohd Yusoff, Khatijah (2005) Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique. Pertanika Journal of Tropical Agricultural Science, 28 (1). pp. 73-77. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2028%20(1)%20Apr.%202005/JTAS%20Vol.28%20(1)%202005%20(73-77).pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A simple and rapid method of DNA microsatellite isolation based on the Random Amplified Microsatellites (RAMs) PCR technique was used in this study. The work presented here is part of a continuous effort in refining and perfecting the technique for more rapid, effective and optimum productivity in single locus microsatellite marker development for the River catfish, Mystus nemurus. The current refined protocol for microsatellite isolation was able to detect a total of 135 microsatellite regions resulting in 42 unique genomic sequences being submitted to GenBank. This refined technique is able to reduce the total time required from peR cloning till sequencing specific microsatellite regions to less than three and a half months.
format Article
author Chan, Soon Choy
Siraj, Siti Shapor
Tan, Soon Guan
Mohd Yusoff, Khatijah
spellingShingle Chan, Soon Choy
Siraj, Siti Shapor
Tan, Soon Guan
Mohd Yusoff, Khatijah
Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique
author_facet Chan, Soon Choy
Siraj, Siti Shapor
Tan, Soon Guan
Mohd Yusoff, Khatijah
author_sort Chan, Soon Choy
title Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique
title_short Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique
title_full Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique
title_fullStr Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique
title_full_unstemmed Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique
title_sort rapid and non-radioactive detection method of microsatellites in mystus nemurus: a refined technique
publisher Universiti Putra Malaysia Press
publishDate 2005
url http://psasir.upm.edu.my/id/eprint/3656/1/Rapid_and_Non-radioactive_Detection_Method_of_Microsatellites_in_Mystus.pdf
http://psasir.upm.edu.my/id/eprint/3656/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2028%20(1)%20Apr.%202005/JTAS%20Vol.28%20(1)%202005%20(73-77).pdf
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