Characterisation and molecular dynamic simulations of J15 asparaginase from Photobacterium sp. strain J15

• Main Authors: Yaacob, Mohd Adilin, Wan Hasan, Wan Atiqah Najiah, Mohamad Ali, Mohd Shukuri, Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Basri, Mahiran, Leow, Thean Chor
• Format: Article
• Language: English
• Published: Polish Biochemical Society & Committee of Biochemistry & Biophysics Polish Academy of Sciences 2014
• Online Access: http://psasir.upm.edu.my/id/eprint/36735/1/Characterisation%20and%20molecular%20dynamic%20simulations%20of%20J15%20asparaginase%20from%20Photobacterium%20sp.pdf; http://psasir.upm.edu.my/id/eprint/36735/; http://www.actabp.pl/#File?./html/4_2014/745.html
• Institution: Universiti Putra Malaysia

Genome mining revealed a 1011 nucleotide-long fragment encoding a type I l-asparaginase (J15 asparaginase) from the halo-tolerant Photobacterium sp. strain J15. The gene was overexpressed in pET-32b (+) vector in E. coli strain Rosetta-gami B (DE3) pLysS and purified using two-step chromatographic m...