Cloning of a novel phytase from an anaerobic rumen bacterium, Mitsuokella jalaludinii, and its expression in Escherichia coli

• Main Authors: Tan, Wan Qin, Phang, Chiun Yee, Sieo, Chin Chin, Yiap, Beow Chin, Wong, Clemente Michael Vui Ling, Abdullah, Norhani, Radu, Son, Ho, Yin Wan
• Format: Article
• Language: English
• Published: Elsevier 2015
• Online Access: http://psasir.upm.edu.my/id/eprint/36797/1/Cloning%20of%20a%20novel%20phytase%20from%20an%20anaerobic%20rumen%20bacterium%2C%20Mitsuokella%20jalaludinii%2C%20and%20its%20expression%20in%20Escherichia%20coli.pdf; http://psasir.upm.edu.my/id/eprint/36797/; http://www.sciencedirect.com/science/article/pii/S2095311914609606
• Institution: Universiti Putra Malaysia

The full length phytase gene of Mitsuokella jalaludinii was successfully cloned and was found to be 1047 bp in length, with 348 amino acids, and was designated as PHY7 phytase gene. A comparison of the sequence of PHY7 phytase gene of M. jalaludinii with various microbial phytase gene sequences show...