Detection and identification of antibiotic biosynthesis genes in Bacillus subtilis strains

Two Bacillus subtilis strains L10 and G1, previously isolated from fermented pickles, were tested for antibacterial activity against Vibrio harveyi using a well-diffusion agar assay. Antibiotic biosynthesis genes were then detected in both bacterial strains and included the following genes: bacA, ba...

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Main Authors: Zokaeifar, Hadi, Babaei, Nahid, Saad, Che Roos, Kamarudin, Mohd Salleh, Sijam, Kamaruzaman, Balcázar, Jose Luis
Format: Article
Language:English
Published: Taylor & Francis 2014
Online Access:http://psasir.upm.edu.my/id/eprint/37006/1/Detection%20and%20identification%20of%20antibiotic%20biosynthesis%20genes%20in%20Bacillus%20subtilis%20strains.pdf
http://psasir.upm.edu.my/id/eprint/37006/
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Institution: Universiti Putra Malaysia
Language: English
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spelling my.upm.eprints.370062015-11-11T06:54:15Z http://psasir.upm.edu.my/id/eprint/37006/ Detection and identification of antibiotic biosynthesis genes in Bacillus subtilis strains Zokaeifar, Hadi Babaei, Nahid Saad, Che Roos Kamarudin, Mohd Salleh Sijam, Kamaruzaman Balcázar, Jose Luis Two Bacillus subtilis strains L10 and G1, previously isolated from fermented pickles, were tested for antibacterial activity against Vibrio harveyi using a well-diffusion agar assay. Antibiotic biosynthesis genes were then detected in both bacterial strains and included the following genes: bacA, bacB and bacD genes for bacilysin production, ppsE/fenB gene for plipastatin/fengycin production, albF and albA genes for subtilosin production, and srfAB and sfP genes for surfactin production. Based on these results, two B. subtilis strains could be considered as potential biological control agents in aquaculture. Taylor & Francis 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37006/1/Detection%20and%20identification%20of%20antibiotic%20biosynthesis%20genes%20in%20Bacillus%20subtilis%20strains.pdf Zokaeifar, Hadi and Babaei, Nahid and Saad, Che Roos and Kamarudin, Mohd Salleh and Sijam, Kamaruzaman and Balcázar, Jose Luis (2014) Detection and identification of antibiotic biosynthesis genes in Bacillus subtilis strains. Biocontrol Science and Technology, 24 (2). pp. 233-240. ISSN 0958-3157: ESSN: 1360-0478 10.1080/09583157.2013.852653
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Two Bacillus subtilis strains L10 and G1, previously isolated from fermented pickles, were tested for antibacterial activity against Vibrio harveyi using a well-diffusion agar assay. Antibiotic biosynthesis genes were then detected in both bacterial strains and included the following genes: bacA, bacB and bacD genes for bacilysin production, ppsE/fenB gene for plipastatin/fengycin production, albF and albA genes for subtilosin production, and srfAB and sfP genes for surfactin production. Based on these results, two B. subtilis strains could be considered as potential biological control agents in aquaculture.
format Article
author Zokaeifar, Hadi
Babaei, Nahid
Saad, Che Roos
Kamarudin, Mohd Salleh
Sijam, Kamaruzaman
Balcázar, Jose Luis
spellingShingle Zokaeifar, Hadi
Babaei, Nahid
Saad, Che Roos
Kamarudin, Mohd Salleh
Sijam, Kamaruzaman
Balcázar, Jose Luis
Detection and identification of antibiotic biosynthesis genes in Bacillus subtilis strains
author_facet Zokaeifar, Hadi
Babaei, Nahid
Saad, Che Roos
Kamarudin, Mohd Salleh
Sijam, Kamaruzaman
Balcázar, Jose Luis
author_sort Zokaeifar, Hadi
title Detection and identification of antibiotic biosynthesis genes in Bacillus subtilis strains
title_short Detection and identification of antibiotic biosynthesis genes in Bacillus subtilis strains
title_full Detection and identification of antibiotic biosynthesis genes in Bacillus subtilis strains
title_fullStr Detection and identification of antibiotic biosynthesis genes in Bacillus subtilis strains
title_full_unstemmed Detection and identification of antibiotic biosynthesis genes in Bacillus subtilis strains
title_sort detection and identification of antibiotic biosynthesis genes in bacillus subtilis strains
publisher Taylor & Francis
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/37006/1/Detection%20and%20identification%20of%20antibiotic%20biosynthesis%20genes%20in%20Bacillus%20subtilis%20strains.pdf
http://psasir.upm.edu.my/id/eprint/37006/
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