Extraction of total RNA from sperm of New Zealand white rabbits for detection of phospholipase C zeta (PLC ζ)

• Main Authors: Samsul Baharil, Nur Hayati, Mohamad Nawi, Muhamad Khalil, Cheah, Yoke Kqueen, Sukardi, Sabrina
• Format: Article
• Language: English
• Published: 2015
• Online Access: http://psasir.upm.edu.my/id/eprint/37597/1/Extraction%20of%20total%20RNA%20from%20sperm%20of%20New%20Zealand%20white%20rabbits%20for%20detection%20of%20phospholipase%20C%20zeta%20%28PLC%20%CE%B6%29.pdf; http://psasir.upm.edu.my/id/eprint/37597/; http://www.journalajst.com/extraction-total-rna-sperm-new-zealand-white-rabbits-detection-phospholipase-c-zeta-plc-%CE%B6
• Institution: Universiti Putra Malaysia

Fertilization is defined as the fusion of a spermatozoon to an ovum. Upon fusion, a sperm specific factor Phospholipase C ζ was inserted triggering long lasting Ca2+ oscillations which drive egg activation and early embryonic development phase. Previous studies indicate that PLCζ homologues have bee...