Effect of glycerol feed in methanol induction phase for hepatitis B surface antigen expression in Pichia pastoris strain KM71

• Main Authors: Morvarid, Akhavan Rezaei, Allaudin, Zeenathul Nazariah, Tam, Yew Joon, Hassan, Zuridah, Mohd Lila, Mohd Azmi, Othman, Azizon
• Format: Article
• Language: English
• Published: Universiti Putra Malaysia Press 2012
• Online Access: http://psasir.upm.edu.my/id/eprint/40425/1/3.%20Effect%20of%20glycerol%20feed%20in%20methanol%20induction%20phase%20for%20hepatitis%20B%20surface%20antigen%20expression%20in%20Pichia%20pastoris%20strain%20KM71.pdf; http://psasir.upm.edu.my/id/eprint/40425/; http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JST%20Vol.%2020%20%281%29%20Jan.%202012/08%20Pg%2031-42.pdf
• Institution: Universiti Putra Malaysia

This study describes expression of HBs Ag in methylotrophic yeast, Pichia Pastoris under alcohol oxidase promoter. A single copy number of HBs Ag gene was transformed into pichia strain of KM 71, a Mutˢ type, by using pA0815 pichia expression vector. The recombinant was cultivated in a shake flask e...