Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum
Diadema setosum (Leske, 1778), is one of the common echinoids widely distributed in the Indo-West Pacific Ocean, where it occurs from the Red Sea, Persian Gulf and the east coast of Africa to Japan, Australia and Malaysia. To investigate the developmental basis of morphological changes in embryos an...
Saved in:
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Iranian Fisheries Science Research Institute
2015
|
Online Access: | http://psasir.upm.edu.my/id/eprint/46412/1/Embryonic%2C%20larval%20and%20juvenile%20development%20of%20tropical%20sea%20urchin%2C%20Diadema%20setosum.pdf http://psasir.upm.edu.my/id/eprint/46412/ http://jifro.ir/article-1-1893-en.html |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universiti Putra Malaysia |
Language: | English |
id |
my.upm.eprints.46412 |
---|---|
record_format |
eprints |
spelling |
my.upm.eprints.464122022-06-20T03:14:18Z http://psasir.upm.edu.my/id/eprint/46412/ Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum Rahman, M. Aminur M. Yusoff, Fatimah Arshad, A. Diadema setosum (Leske, 1778), is one of the common echinoids widely distributed in the Indo-West Pacific Ocean, where it occurs from the Red Sea, Persian Gulf and the east coast of Africa to Japan, Australia and Malaysia. To investigate the developmental basis of morphological changes in embryos and larvae, we documented the ontogeny of D. setosum in a controlled laboratory condition at the Institute of Bioscience, Universiti Putra Malaysia, during July–September, 2012. Matured gametes were obtained from the adult individuals and the eggs fertilized at limited sperm concentration (10-5 dilution). The obtained embryos were then reared at 24-25oC. First cleavage (2-cell), 4-cell, 8-cell, 16-cell, 32-cell and multi-cell (Morulla) stages were attained at 01.20, 02.14, 02.44, 03.09, 03.32 and 03.54 h after fertilization, respectively. Blastulae with a mean length of 111.47±1.88 µm (mean±SD) hatched 08.45 h after sperm entry. Gastrula formed 16.36 h post-fertilization and the archenteron extended constantly, while the ectodermal red-pigmented cells migrated synchronously to the apical plate. The pluteus larva started to feed unicellular algae (Chaetoceros calcitrans) in 2 d, grew continuously, and finally attained metamorphic competence within 35 d after fertilization. Induction of metamorphosis took approximately 1 h 30 min from attachment on the substratum to the complete resorption of larval tissues and the development of complete juvenile structure with adult spines, extended tubefeet and well developed pedicellaria, the whole event usually took place within 1 d post-settlement. The newly formed juvenile (473.16 ± 6.96 µm, n=30) with a complete adult structure then grew on coralline algae to 3-month old juvenile, which represents the “sea urchin seed” for stocking in grow-out culture. This study represents the first successful investigation on embryonic, larval and early juvenile development of D. setosum. The findings would greatly be helpful towards the development of breeding and seed production techniques for aquaculture of sea urchins. Iranian Fisheries Science Research Institute 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46412/1/Embryonic%2C%20larval%20and%20juvenile%20development%20of%20tropical%20sea%20urchin%2C%20Diadema%20setosum.pdf Rahman, M. Aminur and M. Yusoff, Fatimah and Arshad, A. (2015) Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum. Iranian Journal of Fisheries Sciences, 14 (2). pp. 409-424. ISSN 1562-2916; ESSN: 2322-5696 http://jifro.ir/article-1-1893-en.html |
institution |
Universiti Putra Malaysia |
building |
UPM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Putra Malaysia |
content_source |
UPM Institutional Repository |
url_provider |
http://psasir.upm.edu.my/ |
language |
English |
description |
Diadema setosum (Leske, 1778), is one of the common echinoids widely distributed in the Indo-West Pacific Ocean, where it occurs from the Red Sea, Persian Gulf and the east coast of Africa to Japan, Australia and Malaysia. To investigate the developmental basis of morphological changes in embryos and larvae, we documented the ontogeny of D. setosum in a controlled laboratory condition at the Institute of Bioscience, Universiti Putra Malaysia, during July–September, 2012. Matured gametes were obtained from the adult individuals and the eggs fertilized at limited sperm concentration (10-5 dilution). The obtained embryos were then reared at 24-25oC. First cleavage (2-cell), 4-cell, 8-cell, 16-cell, 32-cell and multi-cell (Morulla) stages were attained at 01.20, 02.14, 02.44, 03.09, 03.32 and 03.54 h after fertilization, respectively. Blastulae with a mean length of 111.47±1.88 µm (mean±SD) hatched 08.45 h after sperm entry. Gastrula formed 16.36 h post-fertilization and the archenteron extended constantly, while the ectodermal red-pigmented cells migrated synchronously to the apical plate. The pluteus larva started to feed unicellular algae (Chaetoceros calcitrans) in 2 d, grew continuously, and finally attained metamorphic competence within 35 d after fertilization. Induction of metamorphosis took approximately 1 h 30 min from attachment on the substratum to the complete resorption of larval tissues and the development of complete juvenile structure with adult spines, extended tubefeet and well developed pedicellaria, the whole event usually took place within 1 d post-settlement. The newly formed juvenile (473.16 ± 6.96 µm, n=30) with a complete adult structure then grew on coralline algae to 3-month old juvenile, which represents the “sea urchin seed” for stocking in grow-out culture. This study represents the first successful investigation on embryonic, larval and early juvenile development of D. setosum. The findings would greatly be helpful towards the development of breeding and seed production techniques for aquaculture of sea urchins. |
format |
Article |
author |
Rahman, M. Aminur M. Yusoff, Fatimah Arshad, A. |
spellingShingle |
Rahman, M. Aminur M. Yusoff, Fatimah Arshad, A. Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum |
author_facet |
Rahman, M. Aminur M. Yusoff, Fatimah Arshad, A. |
author_sort |
Rahman, M. Aminur |
title |
Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum |
title_short |
Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum |
title_full |
Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum |
title_fullStr |
Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum |
title_full_unstemmed |
Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum |
title_sort |
embryonic, larval and juvenile development of tropical sea urchin, diadema setosum |
publisher |
Iranian Fisheries Science Research Institute |
publishDate |
2015 |
url |
http://psasir.upm.edu.my/id/eprint/46412/1/Embryonic%2C%20larval%20and%20juvenile%20development%20of%20tropical%20sea%20urchin%2C%20Diadema%20setosum.pdf http://psasir.upm.edu.my/id/eprint/46412/ http://jifro.ir/article-1-1893-en.html |
_version_ |
1736835687043301376 |