Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa)

Thymoquinone (TQ) has been shown to exhibit antitumor properties. Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) was developed to improve the bioavailability and cytotoxicity of TQ. This study was conducted to determine the cytotoxic effects of TQ-NLC on breast cancer (MDA-MB-231 and MCF-...

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Main Authors: Wei, Keat Ng, Saiful Yazan, Latifah, Li, Hua Yap, Wan Abd Ghani, Wan Nor Hafiza, Chee, Wun How, Abdullah, Rasedee
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2015
Online Access:http://psasir.upm.edu.my/id/eprint/46734/1/Thymoquinone-loaded%20nanostructured%20lipid%20carrier%20exhibited%20cytotoxicity%20towards%20breast%20cancer%20cell%20lines%20%28MDA-MB-231%20and%20MCF-7%29%20and%20cervical%20cancer%20cell%20lines%20%28HeLa%20and%20SiHa%29.pdf
http://psasir.upm.edu.my/id/eprint/46734/
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spelling my.upm.eprints.467342018-04-23T00:58:52Z http://psasir.upm.edu.my/id/eprint/46734/ Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa) Wei, Keat Ng Saiful Yazan, Latifah Li, Hua Yap Wan Abd Ghani, Wan Nor Hafiza Chee, Wun How Abdullah, Rasedee Thymoquinone (TQ) has been shown to exhibit antitumor properties. Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) was developed to improve the bioavailability and cytotoxicity of TQ. This study was conducted to determine the cytotoxic effects of TQ-NLC on breast cancer (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa). TQ-NLC was prepared by applying the hot high pressure homogenization technique. The mean particle size of TQ-NLC was 35.66 ± 0.1235 nm with a narrow polydispersity index (PDI) lower than 0.25. The zeta potential of TQ-NLC was greater than −30 mV. Polysorbate 80 helps to increase the stability of TQ-NLC. Differential scanning calorimetry showed that TQ-NLC has a melting point of 56.73°C, which is lower than that of the bulk material. The encapsulation efficiency of TQ in TQ-NLC was 97.63 ± 0.1798% as determined by HPLC analysis. TQ-NLC exhibited antiproliferative activity towards all the cell lines in a dose-dependent manner which was most cytotoxic towards MDA-MB-231 cells. Cell shrinkage was noted following treatment of MDA-MB-231 cells with TQ-NLC with an increase of apoptotic cell population (P < 0.05). TQ-NLC also induced cell cycle arrest. TQ-NLC was most cytotoxic towards MDA-MB-231 cells. It induced apoptosis and cell cycle arrest in the cells. Hindawi Publishing Corporation 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46734/1/Thymoquinone-loaded%20nanostructured%20lipid%20carrier%20exhibited%20cytotoxicity%20towards%20breast%20cancer%20cell%20lines%20%28MDA-MB-231%20and%20MCF-7%29%20and%20cervical%20cancer%20cell%20lines%20%28HeLa%20and%20SiHa%29.pdf Wei, Keat Ng and Saiful Yazan, Latifah and Li, Hua Yap and Wan Abd Ghani, Wan Nor Hafiza and Chee, Wun How and Abdullah, Rasedee (2015) Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa). BioMed Research International. art. no. 263131. pp. 1-10. ISSN 2314-6133; ESSN: 2314-6141 10.1155/2015/263131
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Thymoquinone (TQ) has been shown to exhibit antitumor properties. Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) was developed to improve the bioavailability and cytotoxicity of TQ. This study was conducted to determine the cytotoxic effects of TQ-NLC on breast cancer (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa). TQ-NLC was prepared by applying the hot high pressure homogenization technique. The mean particle size of TQ-NLC was 35.66 ± 0.1235 nm with a narrow polydispersity index (PDI) lower than 0.25. The zeta potential of TQ-NLC was greater than −30 mV. Polysorbate 80 helps to increase the stability of TQ-NLC. Differential scanning calorimetry showed that TQ-NLC has a melting point of 56.73°C, which is lower than that of the bulk material. The encapsulation efficiency of TQ in TQ-NLC was 97.63 ± 0.1798% as determined by HPLC analysis. TQ-NLC exhibited antiproliferative activity towards all the cell lines in a dose-dependent manner which was most cytotoxic towards MDA-MB-231 cells. Cell shrinkage was noted following treatment of MDA-MB-231 cells with TQ-NLC with an increase of apoptotic cell population (P < 0.05). TQ-NLC also induced cell cycle arrest. TQ-NLC was most cytotoxic towards MDA-MB-231 cells. It induced apoptosis and cell cycle arrest in the cells.
format Article
author Wei, Keat Ng
Saiful Yazan, Latifah
Li, Hua Yap
Wan Abd Ghani, Wan Nor Hafiza
Chee, Wun How
Abdullah, Rasedee
spellingShingle Wei, Keat Ng
Saiful Yazan, Latifah
Li, Hua Yap
Wan Abd Ghani, Wan Nor Hafiza
Chee, Wun How
Abdullah, Rasedee
Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa)
author_facet Wei, Keat Ng
Saiful Yazan, Latifah
Li, Hua Yap
Wan Abd Ghani, Wan Nor Hafiza
Chee, Wun How
Abdullah, Rasedee
author_sort Wei, Keat Ng
title Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa)
title_short Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa)
title_full Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa)
title_fullStr Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa)
title_full_unstemmed Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa)
title_sort thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (mda-mb-231 and mcf-7) and cervical cancer cell lines (hela and siha)
publisher Hindawi Publishing Corporation
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/46734/1/Thymoquinone-loaded%20nanostructured%20lipid%20carrier%20exhibited%20cytotoxicity%20towards%20breast%20cancer%20cell%20lines%20%28MDA-MB-231%20and%20MCF-7%29%20and%20cervical%20cancer%20cell%20lines%20%28HeLa%20and%20SiHa%29.pdf
http://psasir.upm.edu.my/id/eprint/46734/
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