Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species

• Main Authors: Chan, Soon Choy, Tan, Soon Guan, Siraj, Siti Shapor, Mohd Yusoff, Khatijah
• Format: Article
• Language: English
• Published: Asian-Australasian Association of Animal Production Societies 2005
• Online Access: http://psasir.upm.edu.my/id/eprint/50079/1/Newly%20developed%20microsatellite%20markers%20of%20Mystus%20nemurus%20tested%20for%20cross-species%20amplification%20in%20two%20distantly%20related%20aquacultured%20catfish%20species.pdf; http://psasir.upm.edu.my/id/eprint/50079/; http://www.ajas.info/journal/view.php?number=21149
• Institution: Universiti Putra Malaysia

The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus nemurus were successfully cross-amplified in two distantly related catfish species within the suborder Siluroidei. Five out of the 19 loci that successfully cross-amplified in Pangasius micronemus were polymorphic, while for Clarias batrachus, cross-amplification was successful using 17 polymorphic loci. The observed heterozygosities were high for all the three catfishes. The results indicated that microsatellite loci could be as polymorphic in non-source species as in the source species.