Immobilization of cyclodextringlucanotransferase from Bacillus maceranson bleached kenafbast micro-fibre

Enzyme immobilization is the process of enzyme attachment on solid support that enables repetitive use of enzyme. In this study, kenafbast fibre was bleached with acidified sodium chloriteuntil7 to 13μm in diameter. Then it was functionalized with hexamethylenediamineandglutaraldehyde to form spacer...

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Main Authors: Ng, Lin Cieh, Sulaiman, Safwan, Mokhtar, Mohd Noriznan, Naim, Mohd Nazli, Baharuddin, Azhari Samsu
Format: Conference or Workshop Item
Language:English
Published: Faculty of Engineering, Universiti Putra Malaysia 2016
Online Access:http://psasir.upm.edu.my/id/eprint/50120/1/16.pdf
http://psasir.upm.edu.my/id/eprint/50120/
http://cafei.upm.edu.my/home.php?&value=2016
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Institution: Universiti Putra Malaysia
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spelling my.upm.eprints.501202017-02-01T05:06:44Z http://psasir.upm.edu.my/id/eprint/50120/ Immobilization of cyclodextringlucanotransferase from Bacillus maceranson bleached kenafbast micro-fibre Ng, Lin Cieh Sulaiman, Safwan Mokhtar, Mohd Noriznan Naim, Mohd Nazli Baharuddin, Azhari Samsu Enzyme immobilization is the process of enzyme attachment on solid support that enables repetitive use of enzyme. In this study, kenafbast fibre was bleached with acidified sodium chloriteuntil7 to 13μm in diameter. Then it was functionalized with hexamethylenediamineandglutaraldehyde to form spacer arm and ligand, respectively. Cyclodextringlucanotransferase (CGTase) from Bacillus maceranswas immobilized viacouplinginteraction with ligand onmicrofibre surface. The results show that protein loading and enzyme activity recovery were 27.60% and 21.19%, respectively when 54.196 U/ml of CGTase was used. Besides, storage stability of the immobilized CGTase at 4°C, 25°C and 60°C resulted in 60%, 40% and 15% from its initial activity after 15 days storage. The optimum operating temperature shifted from 60°C to 70°C compared to free CGTase. For reusability study, the immobilized CGTase was able to retain 33% from its initial activity after 7 cycles of batch reaction. Therefore, bleached kenafbast microfibre has high potential to be applied in enzyme immobilization. This can contribute in building sustainable environment as less energy is needed for enzymatic processes in industries. Problems such as effluent disposal and wastage of enzymes can also be eliminated by using the immobilized enzymes developed in this study. Faculty of Engineering, Universiti Putra Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/50120/1/16.pdf Ng, Lin Cieh and Sulaiman, Safwan and Mokhtar, Mohd Noriznan and Naim, Mohd Nazli and Baharuddin, Azhari Samsu (2016) Immobilization of cyclodextringlucanotransferase from Bacillus maceranson bleached kenafbast micro-fibre. In: 3rd International Conference on Agricultural and Food Engineering (CAFEi 2016), 23-25 Aug. 2016, Seri Pacific Hotel, Kuala Lumpur, Malaysia. (pp. 232-237). http://cafei.upm.edu.my/home.php?&value=2016
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Enzyme immobilization is the process of enzyme attachment on solid support that enables repetitive use of enzyme. In this study, kenafbast fibre was bleached with acidified sodium chloriteuntil7 to 13μm in diameter. Then it was functionalized with hexamethylenediamineandglutaraldehyde to form spacer arm and ligand, respectively. Cyclodextringlucanotransferase (CGTase) from Bacillus maceranswas immobilized viacouplinginteraction with ligand onmicrofibre surface. The results show that protein loading and enzyme activity recovery were 27.60% and 21.19%, respectively when 54.196 U/ml of CGTase was used. Besides, storage stability of the immobilized CGTase at 4°C, 25°C and 60°C resulted in 60%, 40% and 15% from its initial activity after 15 days storage. The optimum operating temperature shifted from 60°C to 70°C compared to free CGTase. For reusability study, the immobilized CGTase was able to retain 33% from its initial activity after 7 cycles of batch reaction. Therefore, bleached kenafbast microfibre has high potential to be applied in enzyme immobilization. This can contribute in building sustainable environment as less energy is needed for enzymatic processes in industries. Problems such as effluent disposal and wastage of enzymes can also be eliminated by using the immobilized enzymes developed in this study.
format Conference or Workshop Item
author Ng, Lin Cieh
Sulaiman, Safwan
Mokhtar, Mohd Noriznan
Naim, Mohd Nazli
Baharuddin, Azhari Samsu
spellingShingle Ng, Lin Cieh
Sulaiman, Safwan
Mokhtar, Mohd Noriznan
Naim, Mohd Nazli
Baharuddin, Azhari Samsu
Immobilization of cyclodextringlucanotransferase from Bacillus maceranson bleached kenafbast micro-fibre
author_facet Ng, Lin Cieh
Sulaiman, Safwan
Mokhtar, Mohd Noriznan
Naim, Mohd Nazli
Baharuddin, Azhari Samsu
author_sort Ng, Lin Cieh
title Immobilization of cyclodextringlucanotransferase from Bacillus maceranson bleached kenafbast micro-fibre
title_short Immobilization of cyclodextringlucanotransferase from Bacillus maceranson bleached kenafbast micro-fibre
title_full Immobilization of cyclodextringlucanotransferase from Bacillus maceranson bleached kenafbast micro-fibre
title_fullStr Immobilization of cyclodextringlucanotransferase from Bacillus maceranson bleached kenafbast micro-fibre
title_full_unstemmed Immobilization of cyclodextringlucanotransferase from Bacillus maceranson bleached kenafbast micro-fibre
title_sort immobilization of cyclodextringlucanotransferase from bacillus maceranson bleached kenafbast micro-fibre
publisher Faculty of Engineering, Universiti Putra Malaysia
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/50120/1/16.pdf
http://psasir.upm.edu.my/id/eprint/50120/
http://cafei.upm.edu.my/home.php?&value=2016
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