Detection of leptospires from experimentally infected hamsters by the polymerase chain reaction assay

Leptospirosis outbreaks in humans in Malaysia have been frequently reported in 2010-2011. A study has managed to isolate several pathogenic strains from east region of Peninsular Malaysia. An isolate was inoculated into 21 hamsters to determine the pathogenicity of the isolate. The movement of the i...

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Main Authors: A. Rashid, Fairuz Ridzlan, Bahaman, Abdul Rani, Bejo, Siti Khairani, Mutalib, Abdul Rahim
Format: Conference or Workshop Item
Language:English
Published: 2012
Online Access:http://psasir.upm.edu.my/id/eprint/50222/1/2-6.pdf
http://psasir.upm.edu.my/id/eprint/50222/
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Institution: Universiti Putra Malaysia
Language: English
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spelling my.upm.eprints.502222017-02-02T05:00:56Z http://psasir.upm.edu.my/id/eprint/50222/ Detection of leptospires from experimentally infected hamsters by the polymerase chain reaction assay A. Rashid, Fairuz Ridzlan Bahaman, Abdul Rani Bejo, Siti Khairani Mutalib, Abdul Rahim Leptospirosis outbreaks in humans in Malaysia have been frequently reported in 2010-2011. A study has managed to isolate several pathogenic strains from east region of Peninsular Malaysia. An isolate was inoculated into 21 hamsters to determine the pathogenicity of the isolate. The movement of the isolate was traced and it was detected at Day 3 post infection to be in the kidneys. The use of PCR assay is a reliable method to detect leptospires in the experimental animals and therefore in naturally infected animals as well. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/50222/1/2-6.pdf A. Rashid, Fairuz Ridzlan and Bahaman, Abdul Rani and Bejo, Siti Khairani and Mutalib, Abdul Rahim (2012) Detection of leptospires from experimentally infected hamsters by the polymerase chain reaction assay. In: International Conference on One Health and 24th VAM Congress 2012, 21-23 Sept. 2012, Marriott Putrajaya. (pp. 311-313).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Leptospirosis outbreaks in humans in Malaysia have been frequently reported in 2010-2011. A study has managed to isolate several pathogenic strains from east region of Peninsular Malaysia. An isolate was inoculated into 21 hamsters to determine the pathogenicity of the isolate. The movement of the isolate was traced and it was detected at Day 3 post infection to be in the kidneys. The use of PCR assay is a reliable method to detect leptospires in the experimental animals and therefore in naturally infected animals as well.
format Conference or Workshop Item
author A. Rashid, Fairuz Ridzlan
Bahaman, Abdul Rani
Bejo, Siti Khairani
Mutalib, Abdul Rahim
spellingShingle A. Rashid, Fairuz Ridzlan
Bahaman, Abdul Rani
Bejo, Siti Khairani
Mutalib, Abdul Rahim
Detection of leptospires from experimentally infected hamsters by the polymerase chain reaction assay
author_facet A. Rashid, Fairuz Ridzlan
Bahaman, Abdul Rani
Bejo, Siti Khairani
Mutalib, Abdul Rahim
author_sort A. Rashid, Fairuz Ridzlan
title Detection of leptospires from experimentally infected hamsters by the polymerase chain reaction assay
title_short Detection of leptospires from experimentally infected hamsters by the polymerase chain reaction assay
title_full Detection of leptospires from experimentally infected hamsters by the polymerase chain reaction assay
title_fullStr Detection of leptospires from experimentally infected hamsters by the polymerase chain reaction assay
title_full_unstemmed Detection of leptospires from experimentally infected hamsters by the polymerase chain reaction assay
title_sort detection of leptospires from experimentally infected hamsters by the polymerase chain reaction assay
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/50222/1/2-6.pdf
http://psasir.upm.edu.my/id/eprint/50222/
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