Production of y-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system

• Main Authors: Lin, Yu Kiat, Show, Pau Loke, Yap, Yee Jiun, Ariff, Arbakariya, Mohammad Annuar, Mohammad Suffian, Lai, Oi Ming, Tang, Teck Kim, Juan, Joon Ching, Ling, Tau Chuan
• Format: Article
• Language: English
• Published: Elsevier 2016
• Online Access: http://psasir.upm.edu.my/id/eprint/53680/1/Production%20of%20%CE%B3-cyclodextrin%20by%20Bacillus%20cereus%20cyclodextrin%20glycosyltransferase%20using%20extractive%20bioconversion%20in%20polymer-salt%20aqueous%20two-phase%20system.pdf; http://psasir.upm.edu.my/id/eprint/53680/; https://www.sciencedirect.com/science/article/pii/S1389172315004016
• Institution: Universiti Putra Malaysia

Aqueous two-phase system (ATPS) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. Extractive bioconversion of gamma-cyclodextrin (γ-CD) from soluble starch with cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) enzyme derived from Bacillus cereus was evaluated using polyethylene glycol (PEG)/potassium phosphate based on ATPS. The optimum condition was attained in the ATPS constituted of 30.0% (w/w) PEG 3000 g/mol and 7.0% (w/w) potassium phosphate. A γ-CD concentration of 1.60 mg/mL with a 19% concentration ratio was recovered after 1 h bioconversion process. The γ-CD was mainly partitioned to the top phase (YT = 81.88%), with CGTase partitioning in the salt-rich bottom phase (KCGTase = 0.51). Repetitive batch processes of extractive bioconversion were successfully recycled three times, indicating that this is an environmental friendly and a cost saving technique for γ-CD production and purification.