Evaluation of cells integrity using different fixation time by scanning electron microscopy

• Main Authors: Mad Yasin, Ida Muryany, Rajab, Nor Fadilah, Md Yasin, Ina Salwany, Ghazali, Ahmad Rohi, Saad, Mohd Zamri, Md Ambia, Kaswandi, Sahalan, Ahmad Zorin, Hing, Hiang Lian
• Format: Article
• Language: English
• Published: International Network for Natural Sciences 2016
• Online Access: http://psasir.upm.edu.my/id/eprint/54207/1/Evaluation%20of%20cells%20integrity%20using%20different%20fixation%20time%20by%20scanning%20electron%20microscopy.pdf; http://psasir.upm.edu.my/id/eprint/54207/; http://www.innspub.net/ijb/evaluation-of-cells-integrity-using-different-fixation-time-by-scanning-electron-microscopy/
• Institution: Universiti Putra Malaysia

The study aims to evaluate the integrity and structural changes of two intestinal cells adhered with Lactobacillus sp. in different fixation time. HT-29 and CCD-18Co intestinal cells with Lactobacillus sp. were fixed with 2.5% glutaraldehyde in 0.1 M phosphate buffer for two, four, six and 12 hours...