Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes

• Main Authors: Firouzamandi, Masoumeh, Moeini, Hassan, Hosseini, Davood, Bejo, Mohd Hair, Omar, Abdul Rahman, Mehrbod, Parvaneh, Ideris, Aini
• Format: Article
• Language: English
• Published: The Korean Society of Veterinary Science 2016
• Online Access: http://psasir.upm.edu.my/id/eprint/54518/1/Improved%20immunogenicity%20of%20Newcastle%20disease%20virus%20inactivated%20vaccine%20.pdf; http://psasir.upm.edu.my/id/eprint/54518/; https://vetsci.org/search.php?where=aview&id=10.4142/jvs.2016.17.1.21&code=0118JVS&vmode=AONLY
• Institution: Universiti Putra Malaysia

The present study describes the development of DNA vaccines using the hemagglutinin-neuraminidase (HN) and fusion (F) genes from AF2240 Newcastle disease virus strain, namely pIRES/HN, pIRES/F and pIRES-F/HN. Transient expression analysis of the constructs in Vero cells revealed the successful expre...