Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential

• Main Authors: Akita, Hironaga, Kimura, Zen‑ichiro, Mohd Yusoff, Mohd Zulkhairi, Nakashima, Nobutaka, Hoshino, Tamotsu
• Format: Article
• Language: English
• Published: SpringerOpen 2016
• Online Access: http://psasir.upm.edu.my/id/eprint/54632/1/Isolation%20and%20characterization.pdf; http://psasir.upm.edu.my/id/eprint/54632/; https://springerplus.springeropen.com/articles/10.1186/s40064-016-2237-y
• Institution: Universiti Putra Malaysia

Microbial degradation of lignin releases fermentable sugars, effective utilization of which could support biofuel production from lignocellulosic biomass. In the present study, a lignin-degrading bacterium was isolated from leaf soil and identified as Burkholderia sp. based on 16S rRNA gene sequencing. This strain was named CCA53, and its lignin-degrading capability was assessed by observing its growth on medium containing alkali lignin or lignin-associated aromatic monomers as the sole carbon source. Alkali lignin and at least eight lignin-associated aromatic monomers supported growth of this strain, and the most effective utilization was observed for p-hydroxybenzene monomers. These findings indicate that Burkholderia sp. strain CCA53 has fragmentary activity for lignin degradation.