Cytotoxic and anti - inflammatory activities of Garcinia xanthochymus extracts on cell lines

• Main Authors: Hamidon, Hanisuhana, Taher, Muhammad, Md Jaffri, Juliana, Tg Zakaria, Tg Muhamad Faris Syafiq, Sulaiman, Wan M. A. W., Susanti, Deny, Ichwan, Solachuddin J. A., Zakaria, Zainul A.
• Format: Article
• Language: English
• Published: Universitas Indonesia 2016
• Online Access: http://psasir.upm.edu.my/id/eprint/55414/1/Cytotoxic%20and%20Anti-inflammatory%20activities%20of%20Garcinia%20xanthochymus%20extracts%20on%20cell%20lines.pdf; http://psasir.upm.edu.my/id/eprint/55414/
• Institution: Universiti Putra Malaysia

Objective: Garcinia xanthochymus extract has been reported to have several pharmacological properties. This study was conducted to evaluate cytotoxic and anti-inflammatory activities of G. xanthochymus extracts on cell lines. Methods: The roots and stem barks of plant were extracted using maceration method with n-hexane, dichloromethane and methanol, successively. Cytotoxic activity of the extracts was tested against MCF-7 breast adenocarcinoma using MTT assay. Anti-inflammatory study was evaluated using RAW 264.7 mouse macrophage cells. The nitric oxide production in LPS-stimulated cells was measured using Griess reagent. Results: The results of cytotoxic and anti-inflammatory study showed that dichloromethane and n-hexane extracts of root and stem bark exhibited cytotoxic activity in dose-dependent manner. Meanwhile, for anti-inflammatory study, all root extracts together with stem bark dichloromethane and n-hexane extracts reduce NO production in LPS-stimulated cells in dose dependent manner. Conclusions: This finding indicated that G. xanthochymus extracts might become interesting candidate for treatment of cancer and inflammation.