The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract

• Main Authors: Ling, Tau Chuan, Lyddiatt, Andrew
• Format: Article
• Language: English
• Published: Malaysian Society for Molecular Biology and Biotechnology 2005
• Online Access: http://psasir.upm.edu.my/id/eprint/58492/1/The%20comparative%20recovery%20performance%20of%20anion%20exchange%20and%20dye-ligand%20fluidised%20bed%20adsorption%20of%20G3PDH%20from%20unclarified%20yeast%20extract.pdf; http://psasir.upm.edu.my/id/eprint/58492/; http://www.msmbb.org.my/apjmbb/html13/13e.htm
• Institution: Universiti Putra Malaysia

The comparative recovery performance of anion exchange and dye ligand fluidised bed adsorption of intracellular enzyme, glyceraldehydes 3-phosphate dehydrogenase (G3PDH) from unclarified disrupted yeast has been undertaken. The commercially available anion exchanger, Streamline QXL and the kiesleguhr-agarose composite adsorbent, Microsorb K6AX derived with dye-ligand (Cibaron Blue 3GA) were employed in fluidized bed experiments. The adsorbents were evaluated in respect of recovery performance in terms of yield, purity and enzyme specific activity.