SDS-PAGE and enzyme immunoassay methods for detection of porcine gelatin in edible bird's nest
Porcine gelatin is one of the common adulterant found in processed edible bird’s nest (EBN); as to increase its net weight prior to sale. This study aimed to develop a sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and an enzyme immunoassay method to detect the presence of por...
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| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Halal Products Research Institute, Universiti Putra Malaysia
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/60502/1/Mihrec%204.pdf http://psasir.upm.edu.my/id/eprint/60502/ |
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| Institution: | Universiti Putra Malaysia |
| Language: | English |
| Summary: | Porcine gelatin is one of the common adulterant found in processed edible bird’s nest (EBN); as to increase its net weight prior to sale. This study aimed to develop a sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and an enzyme immunoassay method to detect the presence of porcine gelatin in processed EBN. SDS-PAGE method was developed by comparing the polypeptide patterns of porcine gelatin with the EBN. In the evaluation of the spiked samples of 2.5% to 50% (v/v), SDS-PAGE was able to detect 20% of porcine gelatin in EBN. An indirect enzyme linked immunosorbent assay (ELISA) was then developed for detection of lower amounts of porcine gelatin. A polyclonal rabbit antibodies was raised against the species-specific sequence of porcine collagen alpha 2 (I) chain. The detection limit for porcine gelatin was 0.05% in EBN. This ELISA is suitable for monitoring of EBN’s quality and purity in the supply chain. |
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