Elucidating substrate utilization in biohydrogen production from palm oil mill effluent by Escherichia coli

The present work aims to elucidate substrate utilization from palm oil mill effluent (POME) for biohydrogen production. The experiment was performed in 150 mL serum bottles and the cultures were supplemented with autoclaved-pretreated POME or 0.05 M individual technical grade substrates to investiga...

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Main Authors: Taifor, Azam Fikri, Zakaria, Mohd Rafein, Mohd Yusoff, Mohd Zulkhairi, Toshinari, Maeda, Hassan, Mohd Ali, Shirai, Yoshihito
Format: Article
Language:English
Published: Elsevier 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61703/1/Elucidating%20substrate%20utilization%20in%20biohydrogen%20production%20.pdf
http://psasir.upm.edu.my/id/eprint/61703/
http://www.prims.upm.edu.my/pub/dokumen/pub_journal/201712181324351-s2.0-S0360319916335212-main.pdf , http://www.prims.upm.edu.my/
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Institution: Universiti Putra Malaysia
Language: English
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spelling my.upm.eprints.617032019-01-08T01:32:06Z http://psasir.upm.edu.my/id/eprint/61703/ Elucidating substrate utilization in biohydrogen production from palm oil mill effluent by Escherichia coli Taifor, Azam Fikri Zakaria, Mohd Rafein Mohd Yusoff, Mohd Zulkhairi Toshinari, Maeda Hassan, Mohd Ali Shirai, Yoshihito The present work aims to elucidate substrate utilization from palm oil mill effluent (POME) for biohydrogen production. The experiment was performed in 150 mL serum bottles and the cultures were supplemented with autoclaved-pretreated POME or 0.05 M individual technical grade substrates to investigate the potential use of POME and substrates in preference towards biohydrogen production. The cultures were incubated at 37 °C for 24 h with mild agitation. The maximum hydrogen yield (MHY) obtained was 0.66 mol H2/mol total monomeric sugars and productivity of 3551 μmol/1010 cfu were obtained from engineered Escherichia coli. The POME oligomeric sugars were not metabolized further, which render insignificant conversion of carbohydrates into hydrogen from POME. The yield of hydrogen production increased by 3.5 folds by engineered E. coli BW25113 compared to wild type E. coli BW25113. The preference of the substrates for biohydrogen production is in the following order; glucose > fructose > formic acid. Elsevier 2017-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61703/1/Elucidating%20substrate%20utilization%20in%20biohydrogen%20production%20.pdf Taifor, Azam Fikri and Zakaria, Mohd Rafein and Mohd Yusoff, Mohd Zulkhairi and Toshinari, Maeda and Hassan, Mohd Ali and Shirai, Yoshihito (2017) Elucidating substrate utilization in biohydrogen production from palm oil mill effluent by Escherichia coli. International Journal of Hydrogen Energy, 42 (9). 5812 - 5819. ISSN 0360-3199; ESSN: 1879-3487 http://www.prims.upm.edu.my/pub/dokumen/pub_journal/201712181324351-s2.0-S0360319916335212-main.pdf , http://www.prims.upm.edu.my/ 10.1016/j.ijhydene.2016.11.188
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The present work aims to elucidate substrate utilization from palm oil mill effluent (POME) for biohydrogen production. The experiment was performed in 150 mL serum bottles and the cultures were supplemented with autoclaved-pretreated POME or 0.05 M individual technical grade substrates to investigate the potential use of POME and substrates in preference towards biohydrogen production. The cultures were incubated at 37 °C for 24 h with mild agitation. The maximum hydrogen yield (MHY) obtained was 0.66 mol H2/mol total monomeric sugars and productivity of 3551 μmol/1010 cfu were obtained from engineered Escherichia coli. The POME oligomeric sugars were not metabolized further, which render insignificant conversion of carbohydrates into hydrogen from POME. The yield of hydrogen production increased by 3.5 folds by engineered E. coli BW25113 compared to wild type E. coli BW25113. The preference of the substrates for biohydrogen production is in the following order; glucose > fructose > formic acid.
format Article
author Taifor, Azam Fikri
Zakaria, Mohd Rafein
Mohd Yusoff, Mohd Zulkhairi
Toshinari, Maeda
Hassan, Mohd Ali
Shirai, Yoshihito
spellingShingle Taifor, Azam Fikri
Zakaria, Mohd Rafein
Mohd Yusoff, Mohd Zulkhairi
Toshinari, Maeda
Hassan, Mohd Ali
Shirai, Yoshihito
Elucidating substrate utilization in biohydrogen production from palm oil mill effluent by Escherichia coli
author_facet Taifor, Azam Fikri
Zakaria, Mohd Rafein
Mohd Yusoff, Mohd Zulkhairi
Toshinari, Maeda
Hassan, Mohd Ali
Shirai, Yoshihito
author_sort Taifor, Azam Fikri
title Elucidating substrate utilization in biohydrogen production from palm oil mill effluent by Escherichia coli
title_short Elucidating substrate utilization in biohydrogen production from palm oil mill effluent by Escherichia coli
title_full Elucidating substrate utilization in biohydrogen production from palm oil mill effluent by Escherichia coli
title_fullStr Elucidating substrate utilization in biohydrogen production from palm oil mill effluent by Escherichia coli
title_full_unstemmed Elucidating substrate utilization in biohydrogen production from palm oil mill effluent by Escherichia coli
title_sort elucidating substrate utilization in biohydrogen production from palm oil mill effluent by escherichia coli
publisher Elsevier
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61703/1/Elucidating%20substrate%20utilization%20in%20biohydrogen%20production%20.pdf
http://psasir.upm.edu.my/id/eprint/61703/
http://www.prims.upm.edu.my/pub/dokumen/pub_journal/201712181324351-s2.0-S0360319916335212-main.pdf , http://www.prims.upm.edu.my/
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