Enhancement of thiamine biosynthesis in oil palm seedlings by colonization of endophytic fungus Hendersonia toruloidea

Thiamine, or vitamin B1 plays an indispensable role as a cofactor in crucial metabolic reactions including glycolysis, pentose phosphate pathway and the tricarboxylic acid cycle in all living organisms. Thiamine has been shown to play a role in plant adaptation toward biotic and abiotic stresses. Th...

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Main Authors: Kamarudin, Amirah Nor, Lai, Kok Song, Lamasudin, Dhilia Udie, Abu Seman, Idris, Balia Yusof, Zetty Norhana
Format: Article
Language:English
Published: Frontiers Media 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61731/1/Enhancement%20of%20thiamine%20biosynthesis%20in%20oil%20palm%20seedlings.pdf
http://psasir.upm.edu.my/id/eprint/61731/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651052/
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Institution: Universiti Putra Malaysia
Language: English
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spelling my.upm.eprints.617312019-01-11T03:41:12Z http://psasir.upm.edu.my/id/eprint/61731/ Enhancement of thiamine biosynthesis in oil palm seedlings by colonization of endophytic fungus Hendersonia toruloidea Kamarudin, Amirah Nor Lai, Kok Song Lamasudin, Dhilia Udie Abu Seman, Idris Balia Yusof, Zetty Norhana Thiamine, or vitamin B1 plays an indispensable role as a cofactor in crucial metabolic reactions including glycolysis, pentose phosphate pathway and the tricarboxylic acid cycle in all living organisms. Thiamine has been shown to play a role in plant adaptation toward biotic and abiotic stresses. The modulation of thiamine biosynthetic genes in oil palm seedlings was evaluated in response to root colonization by endophytic Hendersonia toruloidea. Seven-month-old oil palm seedlings were inoculated with H. toruloidea and microscopic analyses were performed to visualize the localization of endophytic H. toruloidea in oil palm roots. Transmission electron microscopy confirmed that H. toruloidea colonized cortical cells. The expression of thiamine biosynthetic genes and accumulation of total thiamine in oil palm seedlings were also evaluated. Quantitative real-time PCR was performed to measure transcript abundances of four key thiamine biosynthesis genes (THI4, THIC, TH1, and TPK) on days 1, 7, 15, and 30 in response to H. toruloidea colonization. The results showed an increase of up to 12-fold in the expression of all gene transcripts on day 1 post-inoculation. On days 7, 15, and 30 post-inoculation, the relative expression levels of these genes were shown to be downregulated. Thiamine accumulation was observed on day 7 post-colonization and subsequently decreased until day 30. This work provides the first evidence for the enhancement of thiamine biosynthesis by endophytic colonization in oil palm seedlings. Frontiers Media 2017-10 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61731/1/Enhancement%20of%20thiamine%20biosynthesis%20in%20oil%20palm%20seedlings.pdf Kamarudin, Amirah Nor and Lai, Kok Song and Lamasudin, Dhilia Udie and Abu Seman, Idris and Balia Yusof, Zetty Norhana (2017) Enhancement of thiamine biosynthesis in oil palm seedlings by colonization of endophytic fungus Hendersonia toruloidea. Frontiers in Plant Science, 8. pp. 1-8. ISSN 1664-462X https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651052/ 10.3389/fpls.2017.01799
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Thiamine, or vitamin B1 plays an indispensable role as a cofactor in crucial metabolic reactions including glycolysis, pentose phosphate pathway and the tricarboxylic acid cycle in all living organisms. Thiamine has been shown to play a role in plant adaptation toward biotic and abiotic stresses. The modulation of thiamine biosynthetic genes in oil palm seedlings was evaluated in response to root colonization by endophytic Hendersonia toruloidea. Seven-month-old oil palm seedlings were inoculated with H. toruloidea and microscopic analyses were performed to visualize the localization of endophytic H. toruloidea in oil palm roots. Transmission electron microscopy confirmed that H. toruloidea colonized cortical cells. The expression of thiamine biosynthetic genes and accumulation of total thiamine in oil palm seedlings were also evaluated. Quantitative real-time PCR was performed to measure transcript abundances of four key thiamine biosynthesis genes (THI4, THIC, TH1, and TPK) on days 1, 7, 15, and 30 in response to H. toruloidea colonization. The results showed an increase of up to 12-fold in the expression of all gene transcripts on day 1 post-inoculation. On days 7, 15, and 30 post-inoculation, the relative expression levels of these genes were shown to be downregulated. Thiamine accumulation was observed on day 7 post-colonization and subsequently decreased until day 30. This work provides the first evidence for the enhancement of thiamine biosynthesis by endophytic colonization in oil palm seedlings.
format Article
author Kamarudin, Amirah Nor
Lai, Kok Song
Lamasudin, Dhilia Udie
Abu Seman, Idris
Balia Yusof, Zetty Norhana
spellingShingle Kamarudin, Amirah Nor
Lai, Kok Song
Lamasudin, Dhilia Udie
Abu Seman, Idris
Balia Yusof, Zetty Norhana
Enhancement of thiamine biosynthesis in oil palm seedlings by colonization of endophytic fungus Hendersonia toruloidea
author_facet Kamarudin, Amirah Nor
Lai, Kok Song
Lamasudin, Dhilia Udie
Abu Seman, Idris
Balia Yusof, Zetty Norhana
author_sort Kamarudin, Amirah Nor
title Enhancement of thiamine biosynthesis in oil palm seedlings by colonization of endophytic fungus Hendersonia toruloidea
title_short Enhancement of thiamine biosynthesis in oil palm seedlings by colonization of endophytic fungus Hendersonia toruloidea
title_full Enhancement of thiamine biosynthesis in oil palm seedlings by colonization of endophytic fungus Hendersonia toruloidea
title_fullStr Enhancement of thiamine biosynthesis in oil palm seedlings by colonization of endophytic fungus Hendersonia toruloidea
title_full_unstemmed Enhancement of thiamine biosynthesis in oil palm seedlings by colonization of endophytic fungus Hendersonia toruloidea
title_sort enhancement of thiamine biosynthesis in oil palm seedlings by colonization of endophytic fungus hendersonia toruloidea
publisher Frontiers Media
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61731/1/Enhancement%20of%20thiamine%20biosynthesis%20in%20oil%20palm%20seedlings.pdf
http://psasir.upm.edu.my/id/eprint/61731/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651052/
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