Impact of elevated CO2 on leaf gas exchange, carbohydrates and secondary metabolites accumulation in Labisia pumila benth

Aims: The aim of this study was to investigate different levels of CO 2 availability alters total phenolic and flavonoid, total available carbohydrate (TAC) and to determine how elevated CO 2 influences gas exchange of Labisia pumila seedlings. Study Design: The 3-months Labisia pumila seedlings of...

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Main Authors: Ibrahim, Mohd Hafiz, Jaafar, Hawa Z. E., Mohd Zain, Nurul Amalina
Format: Article
Language:English
Published: Sciencedomain International 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62096/1/Impact%20of%20elevated%20CO2%20on%20leaf%20gas%20exchange%2C%20carbohydrates%20and%20secondary%20metabolites%20accumulation%20in%20Labisia%20pumila%20benth.pdf
http://psasir.upm.edu.my/id/eprint/62096/
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spelling my.upm.eprints.620962019-03-25T08:27:53Z http://psasir.upm.edu.my/id/eprint/62096/ Impact of elevated CO2 on leaf gas exchange, carbohydrates and secondary metabolites accumulation in Labisia pumila benth Ibrahim, Mohd Hafiz Jaafar, Hawa Z. E. Mohd Zain, Nurul Amalina Aims: The aim of this study was to investigate different levels of CO 2 availability alters total phenolic and flavonoid, total available carbohydrate (TAC) and to determine how elevated CO 2 influences gas exchange of Labisia pumila seedlings. Study Design: The 3-months Labisia pumila seedlings of var Alata, var Pumila and var Lanceolata were put under 1 month to acclimatize in a nursery until ready for the treatment. Carbon dioxide enrichment treatments started when seedlings reached 4 months old by exposing them to three levels of CO 2 , viz., ambient CO 2 (400 µmol/mol), twice ambient (800 µmol/mol) and thrice ambient CO 2 (1200 µmol/mol). The split plot 3 x 3 factorial experiment was designed using randomized complete block design with CO 2 levels being the main plot and varieties as the sub-plot replicated Original Research Article Ibrahim et al.; ARRB, 19(6): 1-16, 2017; Article no.ARRB.36673 2 three times. Place and Duration of Study: Ladang 2, Universiti Putra Malaysia Glasshouse complex between July to November 2011. Methodology: The experiment was conducted for 15 weeks. The measurement of photosynthesis was obtained from a closed infra-red gas analyzer LICOR 6400XT Portable Photosynthesis System (IRGA, Licor Inc., USA). Total phenolics and flavonoid were determined using Follin-Ciocalteau reagent and total available carbohydrate using anthrone reagent. Results: It was found that the treatment effects were contributed by CO 2 levels in all weeks measured in leaf gas exchange properties (Net photosynthesis (A), stomatal conductance (g s), transpiration rate (E), intercellular CO 2 (C i) and Instantaneous water use efficiency, WUE). A combination of increases rates of A and E was responsible for enhancement of WUE by 50% in elevated treatment (800 and 1200 µmol/mol). Total available carbohydrate, total phenolics and flavonoid were also influenced by elevated CO 2 in all weeks of measurement. At end of 15 weeks after treatment (WAT), 44% increase in total available carbohydrate had increased total phenolic and flavonoid by 56% and 149% respectively than ambient treatment. At end of 15 WAT It was found, that the photosynthetic capacity of Labisia pumila was enhanced under elevated CO 2 by significantly have higher maximum electron transfer rate, J max and Rubisco CO 2 fixation capacity V cmax than ambient seedlings. Conclusion: In this work, it was observed that the increase in production of total phenolics and flavonoid in L. pumila might be due to increase in production of total available carbohydrate in the present study. The upregulation of photosynthesis in the present study was supported by enhancement of Maximum electron transfer rate, J max and Rubisco CO 2 fixation capacity V cmax than ambient seedlings that showed this plant has high sink strength to cope with high level of CO 2. Sciencedomain International 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62096/1/Impact%20of%20elevated%20CO2%20on%20leaf%20gas%20exchange%2C%20carbohydrates%20and%20secondary%20metabolites%20accumulation%20in%20Labisia%20pumila%20benth.pdf Ibrahim, Mohd Hafiz and Jaafar, Hawa Z. E. and Mohd Zain, Nurul Amalina (2017) Impact of elevated CO2 on leaf gas exchange, carbohydrates and secondary metabolites accumulation in Labisia pumila benth. Annual Research & Review in Biology, 19 (6). art. no. ARRB.36673. pp. 1-16. ISSN 2347-565X https://journals.indexcopernicus.com/search/article?articleId=1689721 10.9734/ARRB/2017/36673
institution Universiti Putra Malaysia
building UPM Library
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country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Aims: The aim of this study was to investigate different levels of CO 2 availability alters total phenolic and flavonoid, total available carbohydrate (TAC) and to determine how elevated CO 2 influences gas exchange of Labisia pumila seedlings. Study Design: The 3-months Labisia pumila seedlings of var Alata, var Pumila and var Lanceolata were put under 1 month to acclimatize in a nursery until ready for the treatment. Carbon dioxide enrichment treatments started when seedlings reached 4 months old by exposing them to three levels of CO 2 , viz., ambient CO 2 (400 µmol/mol), twice ambient (800 µmol/mol) and thrice ambient CO 2 (1200 µmol/mol). The split plot 3 x 3 factorial experiment was designed using randomized complete block design with CO 2 levels being the main plot and varieties as the sub-plot replicated Original Research Article Ibrahim et al.; ARRB, 19(6): 1-16, 2017; Article no.ARRB.36673 2 three times. Place and Duration of Study: Ladang 2, Universiti Putra Malaysia Glasshouse complex between July to November 2011. Methodology: The experiment was conducted for 15 weeks. The measurement of photosynthesis was obtained from a closed infra-red gas analyzer LICOR 6400XT Portable Photosynthesis System (IRGA, Licor Inc., USA). Total phenolics and flavonoid were determined using Follin-Ciocalteau reagent and total available carbohydrate using anthrone reagent. Results: It was found that the treatment effects were contributed by CO 2 levels in all weeks measured in leaf gas exchange properties (Net photosynthesis (A), stomatal conductance (g s), transpiration rate (E), intercellular CO 2 (C i) and Instantaneous water use efficiency, WUE). A combination of increases rates of A and E was responsible for enhancement of WUE by 50% in elevated treatment (800 and 1200 µmol/mol). Total available carbohydrate, total phenolics and flavonoid were also influenced by elevated CO 2 in all weeks of measurement. At end of 15 weeks after treatment (WAT), 44% increase in total available carbohydrate had increased total phenolic and flavonoid by 56% and 149% respectively than ambient treatment. At end of 15 WAT It was found, that the photosynthetic capacity of Labisia pumila was enhanced under elevated CO 2 by significantly have higher maximum electron transfer rate, J max and Rubisco CO 2 fixation capacity V cmax than ambient seedlings. Conclusion: In this work, it was observed that the increase in production of total phenolics and flavonoid in L. pumila might be due to increase in production of total available carbohydrate in the present study. The upregulation of photosynthesis in the present study was supported by enhancement of Maximum electron transfer rate, J max and Rubisco CO 2 fixation capacity V cmax than ambient seedlings that showed this plant has high sink strength to cope with high level of CO 2.
format Article
author Ibrahim, Mohd Hafiz
Jaafar, Hawa Z. E.
Mohd Zain, Nurul Amalina
spellingShingle Ibrahim, Mohd Hafiz
Jaafar, Hawa Z. E.
Mohd Zain, Nurul Amalina
Impact of elevated CO2 on leaf gas exchange, carbohydrates and secondary metabolites accumulation in Labisia pumila benth
author_facet Ibrahim, Mohd Hafiz
Jaafar, Hawa Z. E.
Mohd Zain, Nurul Amalina
author_sort Ibrahim, Mohd Hafiz
title Impact of elevated CO2 on leaf gas exchange, carbohydrates and secondary metabolites accumulation in Labisia pumila benth
title_short Impact of elevated CO2 on leaf gas exchange, carbohydrates and secondary metabolites accumulation in Labisia pumila benth
title_full Impact of elevated CO2 on leaf gas exchange, carbohydrates and secondary metabolites accumulation in Labisia pumila benth
title_fullStr Impact of elevated CO2 on leaf gas exchange, carbohydrates and secondary metabolites accumulation in Labisia pumila benth
title_full_unstemmed Impact of elevated CO2 on leaf gas exchange, carbohydrates and secondary metabolites accumulation in Labisia pumila benth
title_sort impact of elevated co2 on leaf gas exchange, carbohydrates and secondary metabolites accumulation in labisia pumila benth
publisher Sciencedomain International
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/62096/1/Impact%20of%20elevated%20CO2%20on%20leaf%20gas%20exchange%2C%20carbohydrates%20and%20secondary%20metabolites%20accumulation%20in%20Labisia%20pumila%20benth.pdf
http://psasir.upm.edu.my/id/eprint/62096/
https://journals.indexcopernicus.com/search/article?articleId=1689721
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