Improved stability of live attenuated vaccine gdhA derivative Pasteurella multocidaB:2 by freeze drying method for use as animal vaccine

• Main Authors: Oslan, Siti Nur Hazwani, Halim, Murni, Ramle, Nurfahanah Amirah, Saad, Mohd Zamri, Tan, Joo Shun, Kapri, Mohd Rizal, Ariff, Arbakariya
• Format: Article
• Language: English
• Published: Elsevier 2017
• Online Access: http://psasir.upm.edu.my/id/eprint/62119/1/Improved%20stability%20of%20live%20attenuated%20vaccine%20gdhA%20derivative%20Pasteurella%20multocida.pdf; http://psasir.upm.edu.my/id/eprint/62119/; https://www.sciencedirect.com/science/article/pii/S0011224017301980
• Institution: Universiti Putra Malaysia

The efficacy of attenuated strain of gdhA derivative Pasteurella multocidaB:2 mutant as a live vaccine to control haemorrhagic septicaemia (HS) disease in cattle and buffaloes has been demonstrated. In order to use P. multocida B:2 mutant as a commercial product, it is essential to optimise its formulation for high viability and stability of the live cells. The effectiveness of freeze-drying process using different protective agentformulations for improving cells viability was explored. Sugar and nitrogen compounds were used as protective agents in freeze-drying and the capability of these compounds in maintaining the viability of mutant P. multocida B:2 during subsequent storage was investigated. A complete loss in viability of freeze-dried mutant P. multocida B:2 was monthly observed until 6–12 months of storage at −30 °C, 4 °C and 27 °C when nitrogen compound or no protective agent was added. Trehalose and sucrose showed significantly high survival rate of 93–95% immediately after freeze-drying and the viability was retained during the subsequent storage at −30 °C and 4 °C. A smooth cell surface without any cell-wall damage was observed for the cells formulated with trehalose under scanning electron micrograph. This study presented a freeze-drying process generating a dried live attenuated vaccine formulation with high stability for commercial applications.