Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods

• Main Authors: Lin, Hong Reng, Heish, Chao-Wen, Liu, Cheng-Hui, Muduli, Saradaprasan, Li, Hsing-Fen, Higuchi, Akon, Subbiah, Suresh Kumar, Alarfaj, Abdullah A, Munusamy, Murugan A, Hsu, Shih-Tien, Chen, Da-Chung, Benelli, Giovanni, Murugan, Kadarkarai, Cheng, Nai-Chen, Wang, Han-Chow, Wu, Gwo-Jang
• Format: Article
• Published: Nature Publishing 2017
• Online Access: http://psasir.upm.edu.my/id/eprint/62892/; https://www.nature.com/articles/srep40069
• Institution: Universiti Putra Malaysia

Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) co...