Reference gene validation for gene expression normalization in canine osteosarcoma: a geNorm algorithm approach

• Main Authors: Selvarajah, Gayathri Thevi, Bonestroo, Floor A. S., Timmermans Sprang, Elpetra P. M., Kirpensteijn, Jolle, Mol, Jan A.
• Format: Article
• Language: English
• Published: BioMed Central 2017
• Online Access: http://psasir.upm.edu.my/id/eprint/62931/1/Reference%20gene%20validation%20for%20gene%20expression%20normalization%20in%20canine%20osteosarcoma.pdf; http://psasir.upm.edu.my/id/eprint/62931/; https://bmcvetres.biomedcentral.com/track/pdf/10.1186/s12917-017-1281-3
• Institution: Universiti Putra Malaysia

BACKGROUND: Quantitative PCR (qPCR) is a common method for quantifying mRNA expression. Given the heterogeneity present in tumor tissues, it is crucial to normalize target mRNA expression data using appropriate reference genes that are stably expressed under a variety of pathological and experimenta...