Comparison of Sybr Green I, ELISA and conventional agarose gel-based PCR in the detection of infectious bursal disease virus

• Main Authors: Hamzah, Hairul Aini, Omar, Abdul Rahman, Bejo, Mohd Hair, Ideris, Aini
• Format: Article
• Language: English; English
• Published: Elsevier 2008
• Online Access: http://psasir.upm.edu.my/id/eprint/6343/1/Comparison%20of%20Sybr%20Green%20I.pdf; http://psasir.upm.edu.my/id/eprint/6343/; http://dx.doi.org/10.1016/j.micres.2006.08.003
• Institution: Universiti Putra Malaysia

The current available molecular method to detect infectious bursal disease virus (IBDV) is by reverse transcriptase-polymerase chain reaction (RT-PCR). However, the conventional PCR is time consuming, prone to error and less sensitive. In this study, the performances of Sybr Green I real-time PCR, e...