Tight repression of elastase strain K overexpression by PT7 (A1/04/03) shuttle expression system

• Main Authors: Shamsudin, Nurul Hazwani, Wong, Chee Fah, Raja Abdul Rahman, Raja Noor Zaliha, Mohamad Ali, Mohd Shukuri
• Format: Article
• Language: English
• Published: Zibeline International Publishing 2017
• Online Access: http://psasir.upm.edu.my/id/eprint/63574/1/Tight%20repression%20of%20elastase%20strain%20K%20overexpression.pdf; http://psasir.upm.edu.my/id/eprint/63574/
• Institution: Universiti Putra Malaysia

The PT7(A1/O4/O3) is a promoter resulted from construction of O3 and O4 operators into PA1, a promoter derived from coliphage T7, that evidenced lower the occupancy of the promoter by RNA polymerase and thereby increases the repression factor. A new expression system, pTEL, was successfully construc...