Characterization of infectious bronchitis viruses isolated from commercial poultry farms in Malaysia
Infectious bronchitis virus (IBV) primarily causes a highly infectious bronchitis (IB) disease in commercial chickens. Virus isolation and identification is necessary for diagnosis of the disease. A total of 6 IBV isolates from commercial poultry farms in Malaysia were isolated by inoculation in 9 d...
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| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Faculty of Veterinary Medicine, Universiti Putra Malaysia
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/65003/1/PA-4.pdf http://psasir.upm.edu.my/id/eprint/65003/ |
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| Institution: | Universiti Putra Malaysia |
| Language: | English |
| Summary: | Infectious bronchitis virus (IBV) primarily causes a highly infectious bronchitis (IB) disease in commercial chickens. Virus isolation and identification is necessary for diagnosis of the disease. A total of 6 IBV isolates from commercial poultry farms in Malaysia were isolated by inoculation in 9 day-old embryonated SPF eggs. After few passages, the embryos showed typical lesions of IB such as stunted and curled cross with feather dystrophy. The isolated IBV were characterized by RT-PCR followed by DNA sequencing of partial S1 gene. Sequencing and phylogenetic analysis showed that all the IBV isolates can be divided into 2 distinct groups. In group I (IBS037A, IBS037B, IBS051 and IBS052) shared 70% similarity with Group II (IBS130 and IBS131). However, group II viruses were genetically closed to QX-like IBV isolates with similarity of 93%. Meanwhile, Group I that formed a distinct branch of the phylogenetic tree has genetic distance ranged from 30% to 36% with 793/B, Mass-type, Taiwan-type and QX-like viruses. |
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