High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5

• Main Authors: Raja Abdul Rahman, Raja Noor Zaliha, Baharum, Syarul Nataqain, Basri, Mahiran, Salleh, Abu Bakar
• Format: Article
• Language: English
• Published: Elsevier 2005
• Online Access: http://psasir.upm.edu.my/id/eprint/7071/1/High.pdf; http://psasir.upm.edu.my/id/eprint/7071/; http://www.sciencedirect.com/science/article/pii/S0003269705001752
• Institution: Universiti Putra Malaysia

An organic solvent-tolerant S5 lipase was purified by affinity chromatography and anion exchange chromatography. The molecular mass of the lipase was estimated to be 60 kDa with 387 purification fold. The optimal temperature and pH were 45 °C and 9.0, respectively. The purified lipase was stable at...