Secretory expression of thermostable T1 lipase through bacteriocin release protein

• Main Authors: Raja Abdul Rahman, Raja Noor Zaliha, Leow, Thean Chor, Basri, Mahiran, Salleh, Abu Bakar
• Format: Article
• Language: English
• Published: Elsevier 2005
• Online Access: http://psasir.upm.edu.my/id/eprint/7073/1/Secretory%20expression%20of%20thermostable%20T1%20lipase%20through%20bacteriocin%20release%20protein.pdf; http://psasir.upm.edu.my/id/eprint/7073/; http://www.sciencedirect.com/science/article/pii/S1046592805000082
• Institution: Universiti Putra Malaysia

The extracellular production of T1 lipase was performed by co-expression of pJL3 vector encoding bacteriocin release protein in prokaryotic system. Secretory expression was optimized by considering several parameters, including host strains, inducer (IPTG) concentration, media, induction at A600 nm,...