Extractive bioconversion of gamma-cyclodextrin and recycling of cyclodextrin glycosyltransferase in liquid biphasic system using thermo-separating polymer

• Main Authors: Lin, Yu Kiat, Show, Pau Loke, Yap, Yee Jiun, Ariff, Arbakariya, Mohamad Annuar, Mohamad Suffian, Lai, Oi Ming, Ling, Tau Chuan, Ng, Eng Poh
• Format: Article
• Language: English
• Published: Frontiers Media 2018
• Online Access: http://psasir.upm.edu.my/id/eprint/72694/1/Extractive%20bioconversion%20of%20gamma-cyclodextrin%20and%20recycling%20of%20cyclodextrin%20glycosyltransferase%20in%20liquid%20biphasic%20system%20using%20thermo-separating%20polymer.pdf; http://psasir.upm.edu.my/id/eprint/72694/; https://www.frontiersin.org/articles/10.3389/fchem.2018.00448/full
• Institution: Universiti Putra Malaysia

An extractive bioconversion conducted on soluble starch with cyclodextrin glycosyltransferase (CGTase) enzyme in ethylene oxide-propylene oxide (EOPO)/potassium phosphates liquid biphasic system (LBS) to extract gamma-cyclodextrin (γ-CD) was examined. A range of EOPO (with potassium phosphates) molecular weights was screen to investigate the effect of the latter on the partioning efficency of CGTase and γ-CD. The results show that the optimal top phase γ-CD yield (74.4%) was reached in 35.0% (w/w) EOPO 970 and 10.0% (w/w) potassium phosphate with 2.0% (w/w) sodium chloride. A theoretical explanation for the effect of NaCl on γ-CD was also presented. After a 2 h bioconversion process, a total of 0.87 mg/mL concentration of γ-CD was produced in the EOPO/ phosphates LBS top phase. After the extraction of top phase from LBS, four continuous repetitive batches were successfully conducted with relative CGTase activity of 1.00, 0.86, 0.45, and 0.40 respectively.