Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate

Diacylglycerols (DAG) were enzymatically synthesized by lipase-catalyzed esterification of glycerol with fatty acids from corn oil deodorizer distillate (CrODD). Effects of reaction parameters such as reaction time, temperature, enzyme type, enzyme load, substrate mole ratio, and water content, as w...

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Main Authors: Lo, Seong Koon, Baharin, Badlishah Sham, Tan, Chin Ping, Lai, Oi Ming
Format: Article
Language:English
English
Published: Taylor & Francis 2005
Online Access:http://psasir.upm.edu.my/id/eprint/7280/1/Enzyme.pdf
http://psasir.upm.edu.my/id/eprint/7280/
http://dx.doi.org/10.1081/FBT-200035014
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spelling my.upm.eprints.72802015-10-06T00:25:43Z http://psasir.upm.edu.my/id/eprint/7280/ Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate Lo, Seong Koon Baharin, Badlishah Sham Tan, Chin Ping Lai, Oi Ming Diacylglycerols (DAG) were enzymatically synthesized by lipase-catalyzed esterification of glycerol with fatty acids from corn oil deodorizer distillate (CrODD). Effects of reaction parameters such as reaction time, temperature, enzyme type, enzyme load, substrate mole ratio, and water content, as well as the effect of molecular sieves as a water adsorbent were investigated. Rhizomucor miehei lipase (Lipozyme RM IM) was found to be most effective among the lipases screened. The following conditions yielded 70.0% (w/w) DAG: 5 h reaction time, 65°C reaction temperature, 10% (w/w) Lipozyme RM IM, 2.5:1 fatty acid to glycerol molar ratio, and 30% (w/w) molecular sieves. DAG synthesis of 12.4% (w/w) was still observed at 10% (w/w) water content. 84.2% (w/w) of DAG was obtained after purification. The DAG oil comprised predominantly of 1-oleoyl-3-linoleoyl-glycerol (28.5%), 1,3-diolein (22.7%), 1-oleoyl-2-linoleoyl-glycerol (17.9%), and 1,2-diolein (10.9%). Fatty acid profile was similar to that of refined, bleached and deodorised (RBD) corn oil. The ratio of 1,3- to 1,2-positional isomers of DAG was at 1.82:1. Taylor & Francis 2005-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7280/1/Enzyme.pdf Lo, Seong Koon and Baharin, Badlishah Sham and Tan, Chin Ping and Lai, Oi Ming (2005) Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate. Food Biotechnology, 18 (3). pp. 265-278. ISSN 0890-5436 http://dx.doi.org/10.1081/FBT-200035014 10.1081/FBT-200035014 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
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url_provider http://psasir.upm.edu.my/
language English
English
description Diacylglycerols (DAG) were enzymatically synthesized by lipase-catalyzed esterification of glycerol with fatty acids from corn oil deodorizer distillate (CrODD). Effects of reaction parameters such as reaction time, temperature, enzyme type, enzyme load, substrate mole ratio, and water content, as well as the effect of molecular sieves as a water adsorbent were investigated. Rhizomucor miehei lipase (Lipozyme RM IM) was found to be most effective among the lipases screened. The following conditions yielded 70.0% (w/w) DAG: 5 h reaction time, 65°C reaction temperature, 10% (w/w) Lipozyme RM IM, 2.5:1 fatty acid to glycerol molar ratio, and 30% (w/w) molecular sieves. DAG synthesis of 12.4% (w/w) was still observed at 10% (w/w) water content. 84.2% (w/w) of DAG was obtained after purification. The DAG oil comprised predominantly of 1-oleoyl-3-linoleoyl-glycerol (28.5%), 1,3-diolein (22.7%), 1-oleoyl-2-linoleoyl-glycerol (17.9%), and 1,2-diolein (10.9%). Fatty acid profile was similar to that of refined, bleached and deodorised (RBD) corn oil. The ratio of 1,3- to 1,2-positional isomers of DAG was at 1.82:1.
format Article
author Lo, Seong Koon
Baharin, Badlishah Sham
Tan, Chin Ping
Lai, Oi Ming
spellingShingle Lo, Seong Koon
Baharin, Badlishah Sham
Tan, Chin Ping
Lai, Oi Ming
Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate
author_facet Lo, Seong Koon
Baharin, Badlishah Sham
Tan, Chin Ping
Lai, Oi Ming
author_sort Lo, Seong Koon
title Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate
title_short Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate
title_full Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate
title_fullStr Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate
title_full_unstemmed Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate
title_sort enzyme-catalyzed production and chemical composition of diacylglycerols from corn oil deodorizer distillate
publisher Taylor & Francis
publishDate 2005
url http://psasir.upm.edu.my/id/eprint/7280/1/Enzyme.pdf
http://psasir.upm.edu.my/id/eprint/7280/
http://dx.doi.org/10.1081/FBT-200035014
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