Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells

• Main Authors: Mohd Esa, Norhaizan, Muniandy, Katyakyini, Sivapragasam, Gothai, Subbiah, Suresh Kumar, Arulselvan, Palanisamy
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2018
• Online Access: http://psasir.upm.edu.my/id/eprint/73151/1/CANCER.pdf; http://psasir.upm.edu.my/id/eprint/73151/; https://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=8;spage=394;epage=402;aulast=Gothai
• Institution: Universiti Putra Malaysia

Objective: To identify the bioactive extracts from Alternanthera sessilis and investigate its cytotoxicity potential against colon cancer cells, HT-29. Methods: This study examined the effects of three parts (aerial, leaf, stem) of whole plant on HT-29 colon cancer cell lines. Three different extracts from the plant parts were prepared by maceration technique using 80% ethanol. The anticancer activities were determined using MTT, clonogenic, cell motility and AOPI assay. The chemical composition profiling was analyzed by GC-MS. Results: Among three plant part extracts, leaf extract greatly suppressed the growth of colon cancer cells in time and dosage-dependent manner, followed by aerial and stem. The cytotoxicity results were rationalized with clonogenic, cell motility and AO/PI assay, where extract showed the most active activity compared to aerial and stem extracts. GC-MS analysis of leaf extract showed there were various recognized anti-cancer, anti-oxidant and anti-inflammatory compounds. Conclusions: Amid the screened extracts, the leaf extract exhibits the credible cytotoxic, anti-proliferative and apoptotic activity and hence, our findings call for additional research to conclude the active compounds and their mechanisms determining the apoptotic activity.