Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: types of ion exchange adsorbent and grafted polymer architecture

• Main Authors: Hon, Wei Ng, Fu, Micky Xiang Lee, Gek, Kee Chua, Gan, Bee Koon, Tan, Wen Siang, Chien, Wei Ooi, Siah, Ying Tang, Eng, Seng Chan, Beng, Ti Tey
• Format: Article
• Language: English
• Published: Wiley 2018
• Online Access: http://psasir.upm.edu.my/id/eprint/73901/1/Size-selective%20purification%20of%20hepatitis%20B%20virus-like%20particle%20in%20flow-through%20chromatography%20types%20of%20ion%20exchange%20adsorbent%20and%20grafted%20polymer%20architecture.pdf; http://psasir.upm.edu.my/id/eprint/73901/; https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201700823
• Institution: Universiti Putra Malaysia

Hepatitis B virus-like particles expressed in Escherichia coli were purified using anion exchange adsorbents grafted with polymer poly(oligo(ethylene glycol) methacrylate) in flow-through chromatography mode. The virus-like particles were selectively excluded, while the relatively smaller sized host...