Wilting of bean plants from tobacco mosaic virus from smoking tobacco in Pakistan

• Main Authors: Iftikhar, Yasir, Vadamalai, Ganesan, Sajid, Ashara, Mubeen, Mustansar, I. Ullah, Muhammad
• Format: Article
• Published: Pharmaceutical and Biological Society 2018
• Online Access: http://psasir.upm.edu.my/id/eprint/74442/; https://www.ajbls.com/article/2018/7/2/77-80
• Institution: Universiti Putra Malaysia

Tobacco mosaic virus (TMV) was detected in one out of three samples of different commercial brands of cigarettes by RT-PCR. A PCR product between 650-680bp in size was observed on 1% agarose gel, which was confirmed as TMV through sequencing. Mechanical inoculation of sap from the PCR positive sampl...