A thermoalkaliphilic lipase of Geobacillus sp. T1

• Main Authors: Leow, Thean Chor, Raja Abdul Rahman, Raja Noor Zaliha, Basri, Mahiran, Salleh, Abu Bakar
• Format: Article
• Language: English
• Published: Springer 2007
• Online Access: http://psasir.upm.edu.my/id/eprint/7523/1/A%20thermoalkaliphilic%20lipase%20of%20Geobacillus%20sp.pdf; http://psasir.upm.edu.my/id/eprint/7523/; http://link.springer.com/article/10.1007/s00792-007-0069-y?view=classic
• Institution: Universiti Putra Malaysia

A thermoalkaliphilic T1 lipase gene of Geobacillus sp. strain T1 was overexpressed in pGEX vector in the prokaryotic system. Removal of the signal peptide improved protein solubility and promoted the binding of GST moiety to the glutathione-Sepharose column. High-yield purification of T1 lipase was...