Electrochemical DNA biosensor for detection of porcine oligonucleotides using [Ru(bpy)2PIP]2+ complex
An electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) complexruthenium(II) complex, [Ru(bpy)2(PIP)]2+, (bpy = 2,2’bipyridine, PIP = 2-phenylimidazo[4,5-f[[1,10-phenanthroline]) as label redox have been developed. The study was carried out by immobilizatio...
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| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Institute of Research Engineers and Doctors, USA
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/75445/1/Electrochemical%20DNA%20biosensor%20for%20detection%20of%20porcine%20oligonucleotides.pdf http://psasir.upm.edu.my/id/eprint/75445/ https://www.seekdl.org/conferences/paper/details/6076.html |
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| Institution: | Universiti Putra Malaysia |
| Language: | English |
| Summary: | An electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) complexruthenium(II) complex, [Ru(bpy)2(PIP)]2+, (bpy = 2,2’bipyridine, PIP = 2-phenylimidazo[4,5-f[[1,10-phenanthroline]) as label redox have been developed. The study was carried out by immobilization of porcine aminated DNA probes sequences on screen printed electrode (SPE) modified with succinimide-acrylic microspheres and [Ru(bpy)2(PIP)]2+ to detect DNA hybridization event. The electrochemical detection by redox active ruthenium (II) complex was measured by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results indicate that the interaction of [Ru(bpy)2(PIP)]2+ with hybridization complementary DNA has higher response compared to single-stranded and mismatch complementary DNA. Under optimized condition, this porcine DNA biosensor shows linear response range towards target DNA within range of 1 x10 -5 uM to 1x10-13 uM. |
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