Molecular detection of Haemophilus parasuis serotypes 4, 5 or 12 and 13 in Peninsular Malaysia

Although the economic importance of Haemophilus parasuis infection causing Glasser's disease is prevalent throughout pig farms in Peninsular Malaysia, there is a dearth of knowledge on its actual nature. In this study, a multiplex PCR was performed to screen for three major predominant virulent...

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Main Authors: Lee, Chee Yien, Ooi, Peck Toung, Zakaria, Zunita, Mohamed Mustapha, Noordin
Format: Article
Language:English
Published: Malaysian Society of Parasitology and Tropical Medicine 2019
Online Access:http://psasir.upm.edu.my/id/eprint/81408/1/Molecular%20detection%20of%20Haemophilus%20parasuis%20serotypes%204%2C%205%20or%2012%20and%2013%20in%20Peninsular%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/81408/
https://www.cabdirect.org/cabdirect/abstract/20193357569
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Institution: Universiti Putra Malaysia
Language: English
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spelling my.upm.eprints.814082021-02-13T20:02:56Z http://psasir.upm.edu.my/id/eprint/81408/ Molecular detection of Haemophilus parasuis serotypes 4, 5 or 12 and 13 in Peninsular Malaysia Lee, Chee Yien Ooi, Peck Toung Zakaria, Zunita Mohamed Mustapha, Noordin Although the economic importance of Haemophilus parasuis infection causing Glasser's disease is prevalent throughout pig farms in Peninsular Malaysia, there is a dearth of knowledge on its actual nature. In this study, a multiplex PCR was performed to screen for three major predominant virulent strains of H. parasuis, which are serotypes 4, 5 or 12 and 13. A total of 175 tissues or bodily fluid samples of various parts were collected from diseased animals from October, 2016 to February, 2018; with total of 62.9% positive detection of H. parasuis. The highest detection was found to be in the pericardial sac fibrin (90.9%) followed by pleural fibrin, lung, pleural fluid, tonsil, pericardial sac, peritoneal fluid, abdominal fibrin, joint fluid, brain and pericardium. Serotype 13 was the highest (40/110) followed by serotype 4(37/110), serotype 5(31/110) and 12 samples were nontypable (12/110). The presence of untypable serotype also drives to further identification of other serotypes in Malaysia. Malaysian Society of Parasitology and Tropical Medicine 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81408/1/Molecular%20detection%20of%20Haemophilus%20parasuis%20serotypes%204%2C%205%20or%2012%20and%2013%20in%20Peninsular%20Malaysia.pdf Lee, Chee Yien and Ooi, Peck Toung and Zakaria, Zunita and Mohamed Mustapha, Noordin (2019) Molecular detection of Haemophilus parasuis serotypes 4, 5 or 12 and 13 in Peninsular Malaysia. Tropical Biomedicine, 36 (2). pp. 482-487. ISSN 0127-5720 https://www.cabdirect.org/cabdirect/abstract/20193357569
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Although the economic importance of Haemophilus parasuis infection causing Glasser's disease is prevalent throughout pig farms in Peninsular Malaysia, there is a dearth of knowledge on its actual nature. In this study, a multiplex PCR was performed to screen for three major predominant virulent strains of H. parasuis, which are serotypes 4, 5 or 12 and 13. A total of 175 tissues or bodily fluid samples of various parts were collected from diseased animals from October, 2016 to February, 2018; with total of 62.9% positive detection of H. parasuis. The highest detection was found to be in the pericardial sac fibrin (90.9%) followed by pleural fibrin, lung, pleural fluid, tonsil, pericardial sac, peritoneal fluid, abdominal fibrin, joint fluid, brain and pericardium. Serotype 13 was the highest (40/110) followed by serotype 4(37/110), serotype 5(31/110) and 12 samples were nontypable (12/110). The presence of untypable serotype also drives to further identification of other serotypes in Malaysia.
format Article
author Lee, Chee Yien
Ooi, Peck Toung
Zakaria, Zunita
Mohamed Mustapha, Noordin
spellingShingle Lee, Chee Yien
Ooi, Peck Toung
Zakaria, Zunita
Mohamed Mustapha, Noordin
Molecular detection of Haemophilus parasuis serotypes 4, 5 or 12 and 13 in Peninsular Malaysia
author_facet Lee, Chee Yien
Ooi, Peck Toung
Zakaria, Zunita
Mohamed Mustapha, Noordin
author_sort Lee, Chee Yien
title Molecular detection of Haemophilus parasuis serotypes 4, 5 or 12 and 13 in Peninsular Malaysia
title_short Molecular detection of Haemophilus parasuis serotypes 4, 5 or 12 and 13 in Peninsular Malaysia
title_full Molecular detection of Haemophilus parasuis serotypes 4, 5 or 12 and 13 in Peninsular Malaysia
title_fullStr Molecular detection of Haemophilus parasuis serotypes 4, 5 or 12 and 13 in Peninsular Malaysia
title_full_unstemmed Molecular detection of Haemophilus parasuis serotypes 4, 5 or 12 and 13 in Peninsular Malaysia
title_sort molecular detection of haemophilus parasuis serotypes 4, 5 or 12 and 13 in peninsular malaysia
publisher Malaysian Society of Parasitology and Tropical Medicine
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/81408/1/Molecular%20detection%20of%20Haemophilus%20parasuis%20serotypes%204%2C%205%20or%2012%20and%2013%20in%20Peninsular%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/81408/
https://www.cabdirect.org/cabdirect/abstract/20193357569
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