Molecular detection of Haemophilus parasuis serotypes 4, 5 or 12 and 13 in Peninsular Malaysia

• Main Authors: Lee, Chee Yien, Ooi, Peck Toung, Zakaria, Zunita, Mohamed Mustapha, Noordin
• Format: Article
• Language: English
• Published: Malaysian Society of Parasitology and Tropical Medicine 2019
• Online Access: http://psasir.upm.edu.my/id/eprint/81408/1/Molecular%20detection%20of%20Haemophilus%20parasuis%20serotypes%204%2C%205%20or%2012%20and%2013%20in%20Peninsular%20Malaysia.pdf; http://psasir.upm.edu.my/id/eprint/81408/; https://www.cabdirect.org/cabdirect/abstract/20193357569
• Institution: Universiti Putra Malaysia

Although the economic importance of Haemophilus parasuis infection causing Glasser's disease is prevalent throughout pig farms in Peninsular Malaysia, there is a dearth of knowledge on its actual nature. In this study, a multiplex PCR was performed to screen for three major predominant virulent strains of H. parasuis, which are serotypes 4, 5 or 12 and 13. A total of 175 tissues or bodily fluid samples of various parts were collected from diseased animals from October, 2016 to February, 2018; with total of 62.9% positive detection of H. parasuis. The highest detection was found to be in the pericardial sac fibrin (90.9%) followed by pleural fibrin, lung, pleural fluid, tonsil, pericardial sac, peritoneal fluid, abdominal fibrin, joint fluid, brain and pericardium. Serotype 13 was the highest (40/110) followed by serotype 4(37/110), serotype 5(31/110) and 12 samples were nontypable (12/110). The presence of untypable serotype also drives to further identification of other serotypes in Malaysia.