Physiologically relevant alternative carbon sources modulate biofilm formation, cell wall architecture and antifungal resistance of Candida glabrata
Flexibility in carbon metabolism is pivotal for the survival and propagation of many human fungal pathogens within host niches. Indeed, flexible carbon assimilation enhances pathogenicity and affects the immunogenicity of Candida albicans. Over the last decade, Candida glabrata has emerged as one...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81735/1/Physiologically%20relevant%20alternative.pdf http://psasir.upm.edu.my/id/eprint/81735/ https://www.mdpi.com/1422-0067/20/13/3172 |
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| Institution: | Universiti Putra Malaysia |
| Language: | English |
| Summary: | Flexibility in carbon metabolism is pivotal for the survival and propagation of many human
fungal pathogens within host niches. Indeed, flexible carbon assimilation enhances pathogenicity and
affects the immunogenicity of Candida albicans. Over the last decade, Candida glabrata has emerged
as one of the most common and problematic causes of invasive candidiasis. Despite this, the links
between carbon metabolism, fitness, and pathogenicity in C. glabrata are largely unexplored. Therefore,
this study has investigated the impact of alternative carbon metabolism on the fitness and pathogenic
attributes of C. glabrata. We confirm our previous observation that growth on carbon sources other
than glucose, namely acetate, lactate, ethanol, or oleate, attenuates both the planktonic and biofilm
growth of C. glabrata, but that biofilms are not significantly affected by growth on glycerol. We extend
this by showing that C. glabrata cells grown on these alternative carbon sources undergo cell wall
remodeling, which reduces the thickness of their β-glucan and chitin inner layer while increasing
their outer mannan layer. Furthermore, alternative carbon sources modulated the oxidative stress
resistance of C. glabrata as well as the resistance of C. glabrata to an antifungal drug. In short, key fitness
and pathogenic attributes of C. glabrata are shown to be dependent on carbon source. This reaffirms
the perspective that the nature of the carbon sources available within specific host niches is crucial for
C. glabrata pathogenicity during infection. |
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