The influence of serum‐supplemented culture media in a transwell migration assay

• Main Authors: Omar Zaki, Siti Sarah, Kanesan, Livashini, Leong, Ming Yeh Danielle, Vidyadaran, Sharmili
• Format: Article
• Language: English
• Published: Wiley 2019
• Online Access: http://psasir.upm.edu.my/id/eprint/82524/1/The%20influence%20of%20serum.pdf; http://psasir.upm.edu.my/id/eprint/82524/
• Institution: Universiti Putra Malaysia

Our work cautions against the use of serum-supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FBS-supplemented media in the absence of a gradient also resulted in notable microglia migration. Serum can therefore confound the interpretation of a transwell migration assay when another chemoattractant is used.