Xeno-free and feeder-free culture and differentiation of human embryonic stem cells on recombinant vitronectin-grafted hydrogels

• Main Authors: Chen, Li Hua, Sung, Tzu Cheng, Lee, Henry Hsin Chung, Higuchi, Akon, Su, Huan Chiao, Lin, Kuan Ju, Huang, Yu Ru, Ling, Qing Dong, Kumar, Suresh S., Alarfaj, Abdullah A., Murugan, Alwarkurichi Munusamy, Nasu, Michiyo, Chen, Da Chung, Hsu, Shih Tien, Chang, Yung, Lee, Kuei Fang, Wang, Han Chow, Umezawa, Akihiro
• Format: Article
• Language: English
• Published: Royal Society of Chemistry 2019
• Online Access: http://psasir.upm.edu.my/id/eprint/82769/1/Xeno-free%20and%20feeder-free%20culture%20.pdf; http://psasir.upm.edu.my/id/eprint/82769/; https://pubs.rsc.org/en/content/articlelanding/2019/bm/c9bm00418a#!divAbstract
• Institution: Universiti Putra Malaysia

Recombinant vitronectin-grafted hydrogels were developed by adjusting surface charge of the hydrogels with grafting of poly-l-lysine for optimal culture of human embryonic stem cells (hESCs) under xeno- and feeder-free culture conditions, with elasticity regulated by crosslinking time (10–30 kPa), i...